Tag: ammonia

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Emission of ammonia, hydrogen sulfide, and greenhouse gases following application of aluminum sulfate to beef feedlot surfaces

Purpose

Alum has been successfully used in the poultry industry to lower ammonia (NH3) emission from the barns. However, it has not been evaluated to reduce NH3 on beef feedlot surfaces. Additionally, it is not known how it would affect other common emissions from beef feedlot surfaces. The purpose of this study was to determine the effect of adding aluminum sulfate to beef feedlot surfaces on NH3, hydrogen sulfide (H2S), carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O) emissions.

What Did We Do?

Eight feedlot pens (30 animals per pen) at the U.S. Meat Animal Research Center feedlot were utilized. The pens had a central mound constructed on manure and soil and 3 m concrete apron by the feed bunk and cattle were fed a corn-silage based diet. Four pens (30 cattle/pen) had 10% (g g-1) alum applied to the 6 meters immediately behind the concrete bunk apron and four did not receive alum. The amount of alum added to the area was determined on a mass basis for a depth of 5 cm of feedlot surface material (FSM) using the estimated density of feedlot surface material for Nebraska feedlots (1.5 g cm−3). On sampling days, six representative grab samples were collected from the feedlot surface from the six-meter area behind the bunk apron in each pen; samples were combined within pen to make three representative replicates per pen (N=24). Each of the three pooled samples per pen were measured for pH, NH3, H2S, CH4, CO2, and N2O using petri dishes and wind tunnels in an environmental chamber at an ambient temperature of 25°C (77°F) and 50% relative humidity. Flux measurements for NH3, H2S, CH4, CO2, and N2O flux were measured for 15 minutes using Thermo Fisher Scientific 17i, 450i, 55i, 410iQ, and 46i gas analysis instruments, respectively. Samples were analyzed at day -1, 0, 5, 7, 12, 14, 19, 21, and 26.

What Have We Learned?

Addition of alum lowered pH of FSM from 8.3 to 4.8 (p < 0.01) and the pH remained lower in alum-treated pens for 26 days (p < 0.01). Although the pH remained low, NH3 flux was only lower (p < 0.01) at day 0 and day 5 for alum-treated pens compared to the pens with no alum treatment. Nitrous oxide emission was not affected by alum treatment (6.2 vs 5.7 mg m-2 min-1, respectively for 0 and 10% alum treated pens). Carbon dioxide emission was lower for alum-treated pens than non-treated pens from day 5 until the end of the study (p < 0.05), perhaps due to suppressed microbial activity from the lower pH. Hydrogen sulfide emission was higher (p < 0.05) from alum-treated feedlot surface material (0.8 mg m-2 min-1) compared to non-treated feedlot surface material (0.3 mg m-2 min-1), likely due to addition of sulfate with alum. Methane emission was also higher in alum-treated pens (173.6 mg m-2 min-1) than non-treated pens (81.4 mg m-2 min-1). The limited reduction in NH3, along with increased H2S and CH4 emission from the FSM indicates that alum is not a suitable amendment to reduce emissions from beef feedlot surfaces.

Table 1. pH, ammonia (NH3), hydrogen sulfide (H2S), methane (CH4), carbon dioxide (CO2) and nitrous oxide (N2O) emission from feedlot surface material treated with 0 or 10% alum (g g-1 mass basis).
pH NH3
(mg m-2 min-1)		 H2S
(mg m-2 min-1)		 CH4
(mg m-2 min-1)		 CO2
(mg m-2 min-1)		 N2O
(mg m-2 min-1)
Day 0% Alum 10% Alum 0% Alum 10% Alum 0% Alum 10% Alum 0% Alum 10% Alum 0% Alum 10% Alum 0% Alum 10% Alum
-1 8.1 8.3 229.6d 515.9c 0.3 0.4 136.3 x 73.4w 4,542 3,234 3.1 4.2
0 8.3a 4.8b 163.0c 32.4d 0.2 f 1.8 e 43.1 x 193.8w 4,372 5,294 2.9 1.8
5 8.5a 6.3b 279.5c 83.6d 0.4 0.5 84.1 x 309.5w 404y 1,347z 6.0 6.8
7 8.6a 6.7b 120.2 130.0 0.6 f 1.2e 53.4 61.7 468 y 1,903z 15.3 10.9
12 8.6a 7.2b 418.0 320.3 0.3 0.3 104.5 145.7 3,742y 1,939z 3.3 8.0
14 8.9a 7.6b 229.0 145.5 0.2 0.4 25.4x 180.7w 4,203y 2,018z 11.5 9.3
19 8.6a 7.5b 228.0 225.1 0.1 f 1.1e 132.3x 254.7w 5,999y 3,116z 6.9 5.8
21 8.4a 7.2b 232.0 257.0 0.5 0.8 81.9x 250.0w 4,324y 2,477z 2.2 1.9
26 8.6a 8.0b 584.5c 319.9d 0.1f 0.7e 72.2 92.9 5,534y 3,540z 4.7 2.9
Within a parameter and day, different superscripts indicate a significant difference (p < 0.05) between the emissions from the feedlot surface material treated with 0% and 10% alum.

Future Plans

Future research will evaluate the use of aluminum chloride instead of aluminum sulfate to lower pH of FSM and retain nitrogen. Additionally, microbial amendments are being evaluated to determine if they can reduce gaseous emissions from the feedlot surface.

Authors

Presenting author

Mindy J. Spiehs, Research Animal Scientists, USDA ARS Meat Animal Research Center

Corresponding author

Bryan L. Woodbury, Agricultural Engineer, USDA ARS Meat Animal Research Center

Corresponding author email address

bryan.woodbury@usda.gov

Additional Information

For additional information about the use of alum as a feedlot surface amendment, readers are direct to the following: Effects of using aluminum sulfate (alum) as a surface amendment in beef cattle feedlots on ammonia and sulfide emissions. 2022. Sustainability 14(4): 1984 – 2004. https://doi.org/10.3390/su14041984

Acknowledgements

The authors wish to acknowledge USMARC technicians Alan Kruger and Jessie Clark for their assistance with data collection and analysis.

 

The authors are solely responsible for the content of these proceedings. The technical information does not necessarily reflect the official position of the sponsoring agencies or institutions represented by planning committee members, and inclusion and distribution herein does not constitute an endorsement of views expressed by the same. Printed materials included herein are not refereed publications. Citations should appear as follows. EXAMPLE: Authors. 2022. Title of presentation. Waste to Worth. Oregon, OH. April 18-22, 2022. URL of this page. Accessed on: today’s date.

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An Update on Litter Amendments and Ammonia Scrubbers for Reducing Ammonia Emissions and Phosphorus Runoff from Poultry Litter

Purpose

The objectives of the litter amendment research were to determine why alum applications to poultry litter occasionally fail to reduce soluble phosphorus (P) and to determine if aluminum-, calcium- or iron- based nanoparticles would reduce soluble P in litter when applied alone or in combination with conventional litter treatments used for ammonia control, such as alum and/or sodium bisulfate.

The objective of the scrubber research was to design a scrubber that reduces ammonia, dust, and pathogens in the air inside of animal rearing facilities, like broiler houses, rather than the air being exhausted from the facilities. Currently scrubbers are “end of pipe” technology, which purify the exhaust air, so the only economic benefit is the capture of nitrogen, which is relatively inexpensive. Reducing the ammonia, dust, and pathogens in the air inside poultry houses should result in production benefits, such as those found with litter amendments (improved weight gains, better feed conversion, lower susceptibility to disease, and reduced propane use).

What Did We Do?

A series of laboratory studies were conducted with various litter amendments.  The first study was conducted using litter from a commercial broiler house that had been treated with sodium bisulfate ten times over a two year period.  Poultry litter (20 grams) was weighed out into 6 centrifuge tubes and half of the litter samples were treated with alum at a rate of 5% by weight.  The tubes were incubated in the dark for one week, then extracted with 200 ml deionized water for one hour, centrifuged for 15 minutes at 8,000 rpm, filtered through 0.45 um filter paper and analyzed for soluble reactive phosphorus (SRP) using the Murphy-Riley method on an autoanalyzer.

The next four lab studies used the same basic incubation studies, although the litter that was used came from a pen trial we had conducted where we knew the litter had never been treated with sodium bisulfate.  Eighty six different treatment combinations involving conventional ammonia control treatments, such as alum and sodium bisulfate with or without the addition of different types of nanoparticles were used.  The nanoparticles used in this study were: (1) Al-nano – an aluminum based nanoparticle, (2) Fe-nano – an iron based nanoparticle, (3) MNP – a nanoparticle made of both aluminum and iron, and (4) TPX – a calcium silicate based nanoparticle made by N-Clear, Inc.  The sodium bisulfate that was utilized is sold under the tradename PLT (Poultry Litter Treatment) by Jones-Hamilton, Inc.

We also redesigned the ARS Air Scrubber so that it is scrubbing the air inside poultry houses rather than the exhaust air.  The critical design feature to allow this was the use of fast sand filters to remove all particulates from the water and acid used to scrub dust and ammonia, respectively.

What Have We Learned?

We found that alum failed to lower soluble P in poultry litter when the litter had been treated with sodium bisulfate, probably due to the formation of sodium alunite [NaAl3(OH)6(SO4)2], a mineral often found in acid soils where sulfate applications have occurred. The formation of this mineral likely inactivates the Al with respect to P adsorption or precipitation reactions.

We also found that a Ca-based nanoparticle (TPX) was very effective in reducing soluble P in litter, either when applied in combination with alum or sodium bisulfate.  Surprisingly, when TPX was applied with sodium bisulfate at very low levels, the soluble P levels of sodium bisulfate-treated litter decreased from 3,410 mg P/kg (when added alone) to 1,220, 541, and 233 mg P/kg litter, respectively, when 0.25, 0.5, and 1% TPX was added with sodium bisulfate.

Future Plans

We are currently conducting a large pen trial to determine the effect of TPX nanoparticles applied with alum or sodium bisulfate on ammonia emissions, soluble P, and P runoff from small plots using rainfall simulators.

We are also building a full-scale prototype of the indoor ammonia scrubber so that we can begin to test the efficacy of this scrubber.

Author

Philip A. Moore, Jr., Soil Scientist, USDA/ARS, Fayetteville, AR

Philip.Moore@USDA.Gov

Additional Information

Moore, P.A., Jr. 2021. Composition and method for reducing ammonia and soluble phosphorus in runoff and leaching from animal manure. U.S. Patent Application No. 17/171,204. Patent pending.

Moore, P.A., Jr. 2022. A system for removing ammonia, dust and pathogens from air within an animal rearing/sheltering facility. U.S. Patent Application No. 17/715,666.  Patent pending.

 

The authors are solely responsible for the content of these proceedings. The technical information does not necessarily reflect the official position of the sponsoring agencies or institutions represented by planning committee members, and inclusion and distribution herein does not constitute an endorsement of views expressed by the same. Printed materials included herein are not refereed publications. Citations should appear as follows. EXAMPLE: Authors. 2022. Title of presentation. Waste to Worth. Oregon, OH. April 18-22, 2022. URL of this page. Accessed on: today’s date.

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NAEMS: How It Was Done and Lessons Learned

Building Environment and Air Quality – Presented by Al Heber

Development of Draft Emission Estimating Methodologies for AFOs: Process Overview – Presented by Ian Rumsy

National Air Emissions Monitoring Study Status Update – Presented by Bebhinn Do

Purpose

The National Air Emissions Monitoring Study, or NAEMS, was conducted from 2007 – 2010 to gather data to develop scientifically credible methodologies for estimating emissions from animal feeding operations (AFOs). It followed from a 2002 report by the National Academy of Sciences that recommended the development of the emission models. NAEMS was funded by the AFO industry as part of a 2005 voluntary air compliance agreement with the U.S. Environmental Protection Agency (EPA). The goals of the air compliance agreement were to reduce air pollution, monitor AFO emissions, promote a national consensus on emissions estimating methodologies, and ensure compliance with requirements of the Clean Air Act and notification provisions of the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA), and the Emergency Planning and Community Right-to-Know Act (EPCRA). Thus, the design of the study was based both on principles set forth by the National Academy of Sciences and on the needs of EPA and the AFO industry to satisfy the compliance agreement.

What Did We Do

NAEMS monitored barns and lagoons at 25 AFOs in 10 states for approximately 2 years each to measure emissions of ammonia, hydrogen sulfide, particulate matter, and volatile organic compounds. University researchers conducted this monitoring with EPA oversight. The types of AFOs monitored included swine, broiler chickens, egg-laying operations, and dairies. Participating AFOs made their operations available for monitoring for two years and cooperated with the researchers, industry experts, and EPA during the study.

In 2012, EPA used information gathered in NAEMS, along with information provided as part of a 2011 Call for Information, to develop draft emission models for some of the AFO sectors that were monitored. The EPA Science Advisory Board (SAB) conducted a peer review of these original draft emission models and made suggestions for improving the models. Since 2017, EPA began applying the SAB suggestions and developing new draft emission models for each AFO sector. The models estimate farm-scale emissions using information that producers already record or is easy to get (like weather data). The models are not “process-based.” However, the approach aims to estimate emissions from sources based on statistical relationships between air emissions and the meteorological and housing parameters collected that are known to affect processes that generate emissions. The development of process-based models remains a long-term goal of the agency, as we acknowledge process-based models improve the accuracy of emission estimates for the livestock and poultry sectors.

What Have We Learned

During the workshop, panelists will discuss in more detail the lessons learned at various stages of the NAEMS project and how those lessons could inform future work.

Future Plans

The EPA team continues to develop draft emission models using the NAEMS data. It is anticipated that the AFO emission models will be finalized after incorporating input from a stakeholder review period.

Authors

Presenting Authors

    • Albert J. Heber, Professor Emeritus, Agricultural and Biological Engineering
    • Ian C. Rumsey, Physical Scientist, Office of Research & Development, U.S. Environmental Protection Agency
    • Bebhinn Do, Physical Scientist, U.S. Environmental Protection Agency

Corresponding Author

Bebhinn Do, Physical Scientist, U.S. Environmental Protection Agency
do.bebhinn@epa.gov

Additional Information

For updates on NAEMS, please see: https://www.epa.gov/afos-air/national-air-emissions-monitoring-study

Acknowledgements

U.S. Environmental Protection Agency – Office of Research & Development Emission Estimating Methodology development team: Maliha Nash, John Walker, Yijia Dietrich, Carry Croghan

 

The authors are solely responsible for the content of these proceedings. The technical information does not necessarily reflect the official position of the sponsoring agencies or institutions represented by planning committee members, and inclusion and distribution herein does not constitute an endorsement of views expressed by the same. Printed materials included herein are not refereed publications. Citations should appear as follows. EXAMPLE: Authors. 2022. Title of presentation. Waste to Worth. Oregon, OH. April 18-22, 2022. URL of this page. Accessed on: today’s date.

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Impact of sampling timing on measured gas concentrations and emissions at a commercial laying hen house

Purpose

Ammonia and carbon dioxide are two major air pollutants at commercial laying hen houses. Ammonia models are widely used to estimate emissions from individual farms, a region, a country, or the world and assess their potential environmental and ecological impacts. Carbon dioxide models have been used to estimate ventilation rates based on mass balance. Reliable models must be developed based on measurement data from field conditions. However, concentrations and emissions of these two gases vary temporally in layer houses and can affect accuracies of measurement data and emission models. Accuracies of the measurement results are largely affected by instruments and methodologies, which includes sampling timing, i.e., number of samples per day (NSPD) and sampling starting time. The purpose of this study is to demonstrate the impact of measurement timing on ammonia and carbon dioxide concentrations and emissions.

What Did We Do?

A dataset of measured gas concentrations and emissions at a commercial laying hen farm was selected and used as a reference. It contains 5 days of continuous measurement data that were saved every minute. Different sampling timing scenarios were selected based on a literature survey and were applied in a computer simulation. Absolute differences in percentage between the simulation results and the reference were used to assess the effects of sampling timing.

Two sampling timing scenarios were used in this study: (a). sampling at eight different NSPD, i.e., 144, 48, 24, 12, 9, 3, 2, and 1 compared with the continues measurement of 1440 NSPD with equal sampling intervals and the first sampling starting at 8:00 AM; and (b). sampling for the same eight NSPD and equal sampling intervals, but with the first sampling starting at six different times within the respective sampling intervals, including 8:00 AM, compared with the data of 1440 NSPD. For example, when the NSPD was 2, the six starting times were selected at 8:00 AM, 10:00 AM, noon, 2:00 PM, 4:00 PM, and 6:00 PM.

What Have We Learned?

Results demonstrated that, for scenario (a) of the 5 days sampling and measurement (Figure 1), the absolute differences: 1. ranged from 0.02 % (carbon dioxide concentration at 144 NSPD) to 10.04% (Ammonia concentrations at 2 NSPD); 2. was 3.96% for ammonia emissions at 2 NSPD and 6.48% for carbon dioxide emissions at 1 NSPD, both were the largest emission differences; 3. were generally larger in ammonia concentrations than ammonia emissions, but smaller in carbon dioxide concentrations than carbon dioxide emissions; and 4. were generally larger with fewer NSPD for all the four measurement results (ammonia and carbon dioxide concentrations and emissions).

Figure 1. Comparison of average ammonia concentrations (top left), ammonia emission rates (bottom left), carbon dioxide concentrations (top right), and carbon dioxide emission rates (bottom right) with different number of samples per day, starting at 8:00 during a 5-day continuous measurement.

Scenario (b) simulation revealed a new finding that sampling starting times had large impacts on data accuracies as well (Figure 2). The absolute differences 1. ranged from 0.00 % (for both ammonia and carbon dioxide concentrations at 144 NSPD) to 12.92% (ammonia concentrations at 2 NSPD); and 2. was 7.43% for ammonia emissions at 1 NSPD and 7.60% for carbon dioxide emissions at 2 NSPD, both were the largest emission differences. Additionally, scenario (b) demonstrated the same effects as points 3 and 4 in scenario (a).

Figure 2. An example comparison of six different sampling starting times equally distributed within the sampling intervals of 2 hours, at 12 samples per day in the 5 days of sampling on average ammonia concentrations (top left), ammonia emission rates (bottom left) carbon dioxide concentrations (top right), and carbon dioxide emission rates (bottom right).

Future Plans

More research on the effects of sampling timing on gas concentration and emission measurements will be conducted using datasets of longer-term field measurement (> 1 year) with other sampling scenarios based on the literature survey.

Author

Ji-Qin Ni, Professor, Agricultural and Biological Engineering, Purdue University

Corresponding author email address

jiqin@purdue.edu

Additional Information

Wang-Li, L., Q.-F. Li, L. Chai, E. L. Cortus, K. Wang, I. Kilic, B. W. Bogan, J.-Q. Ni, and A. J. Heber. 2013. The National Air Emissions Monitoring Study’s southeast layer site: Part III. Ammonia concentrations and emissions. Transactions of the ASABE. 56(3): 1185-1197.

Ni, J.-Q., S. Liu, C. A. Diehl, T.-T. Lim, B. W. Bogan, L. Chen, L. Chai, K. Wang, and A. J. Heber. 2017. Emission factors and characteristics of ammonia, hydrogen sulfide, carbon dioxide, and particulate matter at two high-rise layer hen houses. Atmospheric Environment. 154: 260-273.

Tong, X., L. Zhao, R. B. Manuzon, M. J. Darr, R. M. Knight, A. J. Heber, and J.-Q. Ni. 2021. Ammonia concentrations and emissions at two commercial manure-belt layer houses with mixed tunnel and cross ventilation. Transactions of ASABE. 64(6): 2073-2087.

Acknowledgements

This work was supported by the USDA National Institute of Food and Agriculture Hatch project 7000907.

 

The authors are solely responsible for the content of these proceedings. The technical information does not necessarily reflect the official position of the sponsoring agencies or institutions represented by planning committee members, and inclusion and distribution herein does not constitute an endorsement of views expressed by the same. Printed materials included herein are not refereed publications. Citations should appear as follows. EXAMPLE: Authors. 2022. Title of presentation. Waste to Worth. Oregon, OH. April 18-22, 2022. URL of this page. Accessed on: today’s date.

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How are Filth Flies Involved in Wasting Nitrogen?

Purpose

Filth flies are species from the Diptera order associated with animal feces and decomposing waste. Beef cattle raised on open pastures are especially susceptible to two species of filth flies: Face flies (Musca autumnalis De Geer) and Horn flies (Haematobia irritans (L.))  because these flies develop exclusively in fresh cattle manure. Filth fly impact on cattle health is related not only to the loss of body weight but also to the transmission of diseases like pink eye and mastitis (Basiel, 2020; Campbell, 1976; Hall, 1984; Nickerson et al., 1995).

Nitrogen losses from cattle’s manure has been reported for domestic flies (Musca domestica) and bottle flies (Neomyia cornicina) (Iwasa et al., 2015; Macqueen & Beirne, 1975). Despite the regular presence of face fly and horn fly in pastures, their effect on the nutrient cycles is little known. The purpose of this study is to understand the relationship between filth fly’s presence in cattle manure with the nitrogen losses caused by an increase in ammonia and nitrous oxide emissions.

What Did We Do?

The study was conducted in four pastures in the Georgia Piedmont: two near Watkinsville and two near Eatonton during June, July, and August of 2021. Ammonia volatilization and nitrous oxide emissions were measured on days 1, 4, 8, and 15 following dung deposition. Manure samples were collected on days 1 and 15. A static chamber was sealed for 24 h on each sampling date to capture manure’s ammonia and nitrous oxide emissions. In each chamber, a glass jar with boric acid was used to trap ammonia, and gas samples were collected. The gas samples were analyzed for nitrous oxide with a Varian Star 3600 CX Gas Chromatograph using an electron capture detector.

The number of filth flies was determined using a net trap covered by a black cloth that was set after 1 min of manure deposition, allowing the flies to oviposit for 10 min. On the days in which ammonia was not measured, a net trap was set to avoid additional oviposits, and record the emergence of filth flies. On the 15th day, we collected the filth flies that emerged from the eggs deposited in the manure during the first day.

What Have We Learned?

We found that cattle’s manure nitrogen loss as nitrous oxide (N2O) and ammonia (NH3) emissions have a direct relationship with the number of horn flies and face flies in the dung, Figure 1. Eighty percent of the flies trapped were horn flies. Dung with less than 5 flies can emit as little as 0.11 mg of N/kg of manure per day, while cattle manure with more than 30 flies can increase this emission by more than 10 times.

Figure 1 Nitrogen emissions such as nitrous oxide and ammonia (mg/kg of manure) and number of filth flies.

Every extra filth fly in manure can increase N emissions by 0.03 mg per kg of manure per day. According to NRCS, 59.1 lbs. of fresh manure is produced by a cow (approx. 1 000 pounds animal) every day (NRCS, 1995). Considering an average of 85 % relative humidity, 4.03 kg of dry manure can be produced per cow day. The actual economic threshold for horn fly is 200 flies per animal (Hogsette et al., 1991; Schreiber et al., 1987), considering a 1 to 1 sex ratio during emergence (Macqueen & Doube, 1988) we are assuming 100 female flies. Since the capacity of horn flies is 8-13 eggs per day (Lysyk, 1999), 100 female horn flies can generate approximately 1,000 new flies every day.  Calculating the nitrogen emissions (4.03 kg of dry manure X 0.03 mg N kg manure x 1,000 flies per day) results in 121 mg of N loss per cow per day when assuming the number of flies is just at the economic threshold. In January of 2022, USDA released the Southern Region Cattle Inventory with a total of 91.9 million head, from which 30.1 million were beef cows (USDA, 2022). Considering the earlier numbers, the horn fly presence in the beef cattle of the Southern Region could be emitting 3,639 kg of Nitrogen to the atmosphere every day.

Future Plans

We will continue the study on ammonia and nitrous oxide emissions under the same conditions during another year to confirm the trends and accuracy of the results. Also, we will implement a study to analyze the effect of the introduction of a parasitic wasp Spalangia endius as a biological control on horn fly and face fly populations and therefore on the manure’s nitrogen losses.

Authors

Presenting author

Natalia B. Espinoza, Research Assistant, Department of Crop and Soil Science, University of Georgia

Corresponding author

Dr. Dorcas H. Franklin, Professor, Department of Crop and Soil Sciences, University of Georgia

Corresponding author email address

dfrankln@uga.edu or dory.franklin@uga.edu

Additional authors

Anish Subedi, Research Assistant, Department of Crop and Soil Science, University of Georgia

Dr. Miguel Cabrera, Professor, Department of Crop and Soil Sciences, University of Georgia

Dr. Nancy Hinkle, Professor, Department of Entomology, University of Georgia

Dr. S. Lawton Stewart, Professor, Department of Animal and Dairy Science, University of Georgia

Additional Information

Basiel, B. (2020). Genomic Evaluation of Horn Fly Resistance and Phenotypes of Cholesterol Deficiency Carriers in Holstein Cattle [PennState University]. Electronic Theses and Dissertations for Graduate Students.

Campbell, J. B. (1976). Effect of Horn Fly Control on Cows as Expressed by Increased Weaning Weights of Calves. Journal of Economic Entomology, 69(6), 711-712. https://doi.org/DOI 10.1093/jee/69.6.711

Hall, R. D. (1984). Relationship of the Face Fly (Diptera, Muscidae) to Pinkeye in Cattle – a Review and Synthesis of the Relevant Literature. Journal of Medical Entomology, 21(4), 361-365. https://doi.org/DOI 10.1093/jmedent/21.4.361

Hogsette, J. A., Prichard, D. L., & Ruff, J. P. (1991). Economic-Effects of Horn Fly (Diptera, Muscidae) Populations on Beef-Cattle Exposed to 3 Pesticide Treatment Regimes. Journal of Economic Entomology, 84(4), 1270-1274. https://doi.org/DOI 10.1093/jee/84.4.1270

Iwasa, M., Moki, Y., & Takahashi, J. (2015). Effects of the Activity of Coprophagous Insects on Greenhouse Gas Emissions from Cattle Dung Pats and Changes in Amounts of Nitrogen, Carbon, and Energy. Environmental Entomology, 44(1), 106-113. https://doi.org/10.1093/ee/nvu023

Lysyk, T. J. (1999). Effect of temperature on time to eclosion and spring emergence of postdiapausing horn flies (Diptera : Muscidae). Environmental Entomology, 28(3), 387-397. https://doi.org/DOI 10.1093/ee/28.3.387

Macqueen, A., & Beirne, B. P. (1975). Influence of Some Dipterous Larvae on Nitrogen Loss from Cattle Dung. Environmental Entomology, 4(6), 868-870. https://doi.org/DOI 10.1093/ee/4.6.868

Macqueen, A., & Doube, B. M. (1988). Emergence, Host-Finding and Longevity of Adult Haematobia-Irritans-Exigua Demeijere (Diptera, Muscidae). Journal of the Australian Entomological Society, 27, 167-174. <Go to ISI>://WOS:A1988P906100002

Nickerson, S. C., Owens, W. E., & Boddie, R. L. (1995). Symposium – Mastitis in Dairy Heifers – Mastitis in Dairy Heifers – Initial Studies on Prevalence and Control. Journal of Dairy Science, 78(7), 1607-1618. https://doi.org/DOI 10.3168/jds.S0022-0302(95)76785-6

NRCS, N. R. C. S. (1995). Animal Manure Management. RCA Publication Archive(7). https://www.nrcs.usda.gov/wps/portal/nrcs/detail/null/?cid=nrcs143_014211

Schreiber, E. T., Campbell, J. B., Kunz, S. E., Clanton, D. C., & Hudson, D. B. (1987). Effects of Horn Fly (Diptera, Muscidae) Control on Cows and Gastrointestinal Worm (Nematode, Trichostrongylidae) Treatment for Calves on Cow and Calf Weight Gains. Journal of Economic Entomology, 80(2), 451-454. https://doi.org/DOI 10.1093/jee/80.2.451

USDA. (2022). Southern Region News Release Cattle Inventory. https://www.nass.usda.gov/Statistics_by_State/Regional_Office/Southern/includes/Publications/Livestock_Releases/Cattle_Inventory/Cattle2022.pdf

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Production of Greenhouse Gases, Ammonia, Hydrogen Sulfide, and Odorous Volatile Organic Compounds from Manure of Beef Feedlot Cattle Implanted with Anabolic Steroids

Animal production is part of a larger agricultural nutrient recycling system that includes soil, water, plants, animals and livestock excreta. When inefficient storage or utilization of nutrients occurs, parts of this cycle become overloaded. The U.S. Beef industry has made great strides in improving production efficiency with a significant emphasis on improving feed efficiency. Improved feed efficiency results in fewer excreted nutrients and volatile organic compounds (VOC) that impair environmental quality. Anabolic steroids are used to improve nutrient feed efficiency which increases nitrogen retention and reduces nitrogen excretion. This study was conducted to determine the methane (CH4), carbon dioxide (CO2), nitrous oxide (N2O), odorous VOCs, ammonia (NH3), and hydrogen sulfide (H2S) production from beef cattle manure and urine when aggressive steroid implants strategies were used instead of moderate implant strategies.

What Did We Do?

Two groups of beef steers (60 animals per group) were implanted using two levels of implants (moderate or aggressive). This was replicated three times, twice with spring-born calves and once with fall-born calves, for a total of 360 animals used during the study. Both moderate and aggressive treatment groups received the same initial implant that contain 80 mg trenbolone acetate and 16 mg estradiol. At second implant, steers in the moderate group received an implant that contained 120 mg trenbolone acetate and 24 mg estradiol, while those in the aggressive group received an implant that contained 200 mg trenbolone acetate and 20 mg estradiol. Urine and feces samples were collected individually from 60 animals that received a moderate implant and 60 animals that received an aggressive implant at each of three sampling dates (Spring and Fall 2017 and Spring 2018). Within each treatment, fresh urine and feces from five animals were mixed together to make a composite sample slurry (2:1 ratio of manure:urine) and placed in a petri dish. There were seven composite mixtures for each treatment at each sampling date. Wind tunnels were used to pull air over the petri dishes. Ammonia, carbon dioxide, and nitrous oxide concentrations were measured using an Innova 1412 Photoacoustic Gas Analyzer. Hydrogen sulfide and methane were measured using a Thermo Fisher Scientific 450i and 55i, respectively. Gas measurements were taken a minimum of six times over 24- to 27-day sampling periods.

What Have We Learned?

Flux of ammonia, hydrogen sulfide, methane, nitrous oxide, and total aromatic volatile organic compounds were significantly lower when an aggressive implant strategy was used compared to a moderate implant strategy. However, the flux of total branched-chained volatile organic compounds from the manure increased when aggressive implants were used compared to moderate implants. Overall, this study suggests that air quality may be improved when an aggressive implant is used in beef feedlot animals.

Table 1. Overall average flux of compounds from manure (urine + feces) from beef feedlot cattle implanted with a moderatea or aggressiveb anabolic steroid.
Hydrogen Sulfide Ammonia Methane Carbon Dioxide Nitrous  Oxide Total Sulfidesc Total SCFAd Total BCFAe Total Aromaticsf
µg m-2 min-1 ——–mg m-2 min-1——–
Moderate 4.0±0.1 2489.7±53.0 117.9±4.0 8795±138 8.6±0.1 0.7±0.1 65.2±6.6 5.9±0.5 2.9±0.3
Aggressive 2.7±0.2 2186.4±46.2 104.0±3.8 8055±101 7.4±0.1 0.8±0.1 63.4±5.7 7.6±0.8 2.1±0.2
P-value 0.01 0.04 0.01 0.01 0.01 0.47 0.83 0.05 0.04
aModerate treatment =  120 mg trenbolone acetate and 24 mg estradiol at second implant; bAggressive treatment = 200 mg trenbolone acetate and 20 mg estradiol at second implant; cTotal sulfides = dimethyldisulfide and dimethyltrisulfide; dTotal straight-chained fatty acids (SCFA) = acetic acid, propionic acid, butyric acid, valeric acid, hexanoic acid, and heptanoic acid;  eTotal branch-chained fatty acids (BCFA) = isobutyric acid and isovaleric acid; fTotal aromatics = phenol, 4-methylphenol, 4-ethylphenol, indole, and skatole

Future Plans
Urine and fecal samples are being evaluated to determine the concentration of steroid residues in the livestock waste and the nutrient content (nitrogen, phosphorus, potassium and sulfur) of the urine and feces.

Authors

mindy.spiehs@ars.usda.gov Mindy J. Spiehs, Research Animal Scientist, USDA ARS Meat Animal Research Center, Clay Center, NE

Bryan L. Woodbury, Agricultural Engineer, USDA ARS Meat Animal Research Center, Clay Center, NE

Kristin E. Hales, Research Animal Scientist, USDA ARS Meat Animal Research Center, Clay Center, NE

Additional Information

Will be included in Proceedings of the 2019 Annual International Meeting of the American Society of Agricultural and Biological Engineers.

USDA is an equal opportunity provider and employer. 

Acknowledgements

The authors wish to thank Alan Kruger, Todd Boman, Bobbi Stromer, Brooke Compton, John Holman, Troy Gramke and the USMARC Cattle Operations Crew for assistance with data collection.

The authors are solely responsible for the content of these proceedings. The technical information does not necessarily reflect the official position of the sponsoring agencies or institutions represented by planning committee members, and inclusion and distribution herein does not constitute an endorsement of views expressed by the same. Printed materials included herein are not refereed publications. Citations should appear as follows. EXAMPLE: Authors. 2019. Title of presentation. Waste to Worth. Minneapolis, MN. April 22-26, 2019. URL of this page. Accessed on: today’s date.

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Production of Greenhouse Gases and Odorous Compounds from Manure of Beef Feedlot Cattle Fed Diets With and Without Ionophores

Ionophores are a type of antibiotics that are used in cattle production to shift ruminal fermentation patterns. They do not kill bacteria, but inhibit their ability to function and reproduce. In the cattle rumen, acetate, propionate, and butyrate are the primary volatile fatty acids produced. It is more energetically efficient for the rumen bacteria to produce acetate and use methane as a hydrogen sink rather than propionate. Ionophores inhibit archaea forcing bacteria to produce propionate and butyrate as hydrogen sinks rather than working symbiotically with methanogens to produce methane as a hydrogen sink. Numerous research studies have demonstrated performance advantages when ionophores are fed to beef cattle, but few have considered potential environmental benefits of feeding ionophores. This study was conducted to determine if concentrations of greenhouse gases, odorous volatile organic compounds (VOC), ammonia, and hydrogen sulfide from beef cattle manure could be reduced when an ionophore was fed to finishing cattle.

What Did We Do?

Four pens of feedlot cattle were fed an ionophore (monensin) and four pens received no ionophore (n=30 animals/pen). Samples were collected six times over a two-month period. A minimum of 20 fresh fecal pads were collected from each feedlot pen at each collection. Samples were mixed within pen and a sub-sample was placed in a small wind-tunnel. Duplicate samples for each pen were analyzed. Ammonia, carbon dioxide (CO2), and nitrous oxide (N2O) concentrations were measured using an Innova 1412 Photoacoustic Gas Analyzer. Hydrogen sulfide (H2S) and methane (CH4) were measured using a Thermo Fisher Scientific 450i and 55i, respectively.

What Have We Learned?

 

Table 1. Overall average concentration of compounds from feces of beef feedlot cattle fed diets with and without monensin.
Hydrogen Sulfide Ammonia Methane Carbon Dioxide Nitrous  Oxide Total Sulfidesa Total  SCFAb Total BCFAc Total Aromaticsd
µg L-1 —————-mg L-1—————-
No Monensin 87.3±2.2 1.0±0.2 4.3±0.1 562.5±2.2 0.4±0.0 233.4±18.3 421.6±81.9 16.8±3.1 83.7±6.4
Monensin 73.9±1.4 1.1±0.2 3.2±0.2 567.1±2.1 0.5±0.0 145.5±10.9 388.9±32.5 20.3±2.3 86.4±5.6
P-value 0.30 0.40 0.01 0.65 0.21 0.01 0.79 0.48 0.75
aTotal sulfides = dimethyldisulfide and dimethyltrisulfide; bTotal straight-chained fatty acids (SCFA) = acetic acid, propionic acid, butyric acid, valeric acid, hexanoic acid, and heptanoic acid;  cTotal branch-chained fatty acids (BCFA) = isobutyric acid and isovaleric acid; dTotal aromatics = phenol, 4-methylphenol, 4-ethylphenol, indole, and skatole

Total CH4 concentration decreased when monensin was fed. Of the VOCs measured, only total sulfide concentration was lower for the manure from cattle fed monensin compared to those not fed monensin. Ammonia, N2O, CO2, H2S, and all other odorous VOC were similar between the cattle fed monensin and those not fed monensin. The results only account for concentration of gases emitted from the manure and do not take into account any urinary contributions, but indicate little reduction in odors and greenhouse gases when monensin was fed to beef finishing cattle.

Future Plans

A study is planned for April – July 2019 to measure odor and gas emissions from manure (urine and feces mixture) from cattle fed with and without monensin. Measurements will also be collected from the feedlot surface of pens with cattle fed with and without monensin.  

Authors

Mindy J. Spiehs, Research Animal Scientist, USDA ARS Meat Animal Research Center, Clay Center, NE

mindy.spiehs@ars.usda.gov

Bryan L. Woodbury, Agricultural Engineer, USDA ARS Meat Animal Research Center, Clay Center, NE

Kristin E. Hales, Research Animal Scientist, USDA ARS Meat Animal Research Center, Clay Center, NE

Additional Information

Dr. Hales also looked at growth performance and E. coli shedding when ionophores were fed to finishing beef cattle. This work is published in Journal of Animal Science.

Hales, K.E., Wells, J., Berry, E.D., Kalchayanand, N., Bono, J.L., Kim, M.S. 2017. The effects of monensin in diets fed to finishing beef steers and heifers on growth performance and fecal shedding of Escherichia coli O157:H7. Journal of Animal Science. 95(8):3738-3744. https://pubmed.ncbi.nlm.nih.gov/28805884/.

USDA is an equal opportunity provider and employer.

Acknowledgements

The authors wish to thank Alan Kruger, Todd Boman, and the USMARC Cattle Operations Crew for assistance with data collection.

The authors are solely responsible for the content of these proceedings. The technical information does not necessarily reflect the official position of the sponsoring agencies or institutions represented by planning committee members, and inclusion and distribution herein does not constitute an endorsement of views expressed by the same. Printed materials included herein are not refereed publications. Citations should appear as follows. EXAMPLE: Authors. 2019. Title of presentation. Waste to Worth. Minneapolis, MN. April 22-26, 2019. URL of this page. Accessed on: today’s date.

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Evaluation of current products for use in deep pit swine manure storage structures for mitigation of odors and reduction of NH3, H2S, and VOC emissions from stored swine manure

The main purpose of this research project is an evaluation of the current products available in the open marketplace for using in deep pit swine manure structure as to their effectiveness in mitigation of odors and reduction of hydrogen sulfide (H2S), ammonia (NH3), 11 odorous volatile organic compounds (VOCs) and greenhouse gas (CO2, methane and nitrous oxide) emissions from stored swine manure. At the end of each trial, hydrogen sulfide and ammonia concentrations are measured during and immediately after the manure agitation process to simulate pump-out conditions. In addition, pit manure additives are tested for their impact on manure properties including solids content and microbial community.

What Did We Do?

Figure 1. Reactor simulates swine manure storage with controlled air flow rates.

We are using 15 reactors simulating swine manure storage (Figure 1) filled with fresh swine manure (outsourced from 3 different farms) to test simultaneously four manure additive products using manufacturer recommended dose for each product. Each product is tested in 3 identical dosages and storage conditions. The testing period starts on Day 0 (application of product following the recommended dosage by manufacturer) with weekly additions of manure from the same type of farm. The headspace ventilation of manure storage is identical and controlled to match pit manure storage conditions. Gas and odor samples from manure headspace are collected weekly. Hydrogen sulfide and ammonia concentrations are measured in real time with portable meters (both are calibrated with high precision standard gases). Headspace samples for greenhouse gases are collected with a syringe and vials, and analyzed with a gas chromatograph calibrated for CO2, methane and nitrous oxide. Volatile organic compounds are collected with solid-phase microextraction probes and analyzed with a gas chromatography-mass spectrometry (Atmospheric Environment 150 (2017) 313-321). Odor samples are collected in 10 L Tedlar bags and analyzed using the olfactometer with triangular forced-choice method (Chemosphere, 221 (2019) 787-783). To agitate the manure for pump-out simulation, top and bottom ‘Manure Sampling Ports’ (Figure 1) are connected to a liquid pump and cycling for 5 min. Manure samples are collected at the start and end of the trial and are analyzed for nitrogen content and bacterial populations.

The effectiveness of the product efficacy to mitigate emissions is estimated by comparing gas and odor emissions from the treated and untreated manure (control). The mixed linear model is used to analyze the data for statistical significance.

What we have learned?

Figure 2. Hydrogen sulfide and ammonia concentration increased greatly during agitation process conducted at the end of trial to simulate manure pump-out conditions and assess the instantaneous release of gases. The shade area is the initial 5 minutes of continuous manure agitation.

U.S. pork industry will have science-based, objectively tested information on odor and gas mitigation products. The industry does not need to waste precious resources on products with unproven or questionable performance record. This work addresses the question of odor emissions holistically by focusing on what changes that are occurring over time in the odor/odorants being emitted and how does the tested additive alter manure properties including the microbial community. Additionally, we tested the hydrogen sulfide and ammonia emissions during the agitation process simulating pump-out conditions. For both gases, the emissions increased significantly as shown in Figure 2. The Midwest is an ideal location for swine production facilities as the large expanse of crop production requires large fertilizer inputs, which allows manure to be valued as a fertilizer and recycled and used to support crop production.

Future Plans

We develop and test sustainable technologies for mitigation of odor and gaseous emissions from livestock operations. This involves lab-, pilot-, and farm-scale testing. We are pursuing advanced oxidation (UV light, ozone, plant-based peroxidase) and biochar-based technologies.

Authors

Baitong Chen, M.S. student, Iowa State University

Jacek A. Koziel*, Prof., Iowa State University (koziel@iastate.edu)

Daniel S. Andersen, Assoc. Prof., Iowa State University

David B. Parker, Ph.D., P.E., USDA-ARS-Bushland

Additional Information

  • Heber et al., Laboratory Testing of Commercial Manure Additives for Swine Odor Control. 2001.
  • Lemay, S., Stinson, R., Chenard, L., and Barber, M. Comparative Effectiveness of Five Manure Pit Additives. Prairie Swine Centre and the University of Saskatchewan.
  • 2017 update – Air Quality Laboratory & Olfactometry Laboratory Equipment – Koziel’s Lab. doi: 10.13140/RG.2.2.29681.99688.
  • Maurer, D., J.A. Koziel. 2019. On-farm pilot-scale testing of black ultraviolet light and photocatalytic coating for mitigation of odor, odorous VOCs, and greenhouse gases. Chemosphere, 221, 778-784; doi: 10.1016/j.chemosphere.2019.01.086.
  • Maurer, D.L, A. Bragdon, B. Short, H.K. Ahn, J.A. Koziel. 2018. Improving environmental odor measurements: comparison of lab-based standard method and portable odour measurement technology. Archives of Environmental Protection, 44(2), 100-107.  doi: 10.24425/119699.
  • Maurer, D., J.A. Koziel, K. Bruning, D.B. Parker. 2017. Farm-scale testing of soybean peroxidase and calcium peroxide for surficial swine manure treatment and mitigation of odorous VOCs, ammonia, hydrogen sulfide emissions. Atmospheric Environment, 166, 467-478. doi: 10.​1016/​j.​atmosenv.​2017.​07.​048.
  • Maurer, D., J.A. Koziel, J.D. Harmon, S.J. Hoff, A.M. Rieck-Hinz, D.S Andersen. 2016. Summary of performance data for technologies to control gaseous, odor, and particulate emissions from livestock operations: Air Management Practices Assessment Tool (AMPAT). Data in Brief, 7, 1413-1429. doi: 10.1016/j.dib.2016.03.070.

Acknowledgments

We are thankful to (1) National Pork Board and Indiana Pork for funding this project (NBP-17-158), (2) cooperating farms for donating swine manure and (3) manufacturers for providing products for testing. We are also thankful to coworkers in Dr. Koziel’s Olfactometry Laboratory and Air Quality Laboratory, especially Dr. Chumki Banik, Hantian Ma, Zhanibek Meiirkhanuly, Lizbeth Plaza-Torres, Jisoo Wi, Myeongseong Lee, Lance Bormann, and Prof. Andrzej Bialowiec.

 

The authors are solely responsible for the content of these proceedings. The technical information does not necessarily reflect the official position of the sponsoring agencies or institutions represented by planning committee members, and inclusion and distribution herein does not constitute an endorsement of views expressed by the same. Printed materials included herein are not refereed publications. Citations should appear as follows. EXAMPLE: Authors. 2019. Title of presentation. Waste to Worth. Minneapolis, MN. April 22-26, 2019. URL of this page. Accessed on: today’s date.

 

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Seasonal and Spatial Variations in Aerial Ammonia Concentrations in Deep Pit Beef Cattle Barns

There are known benefits and challenges to finishing beef cattle under roof. The accumulated manure is typically stored in either a bedded pack (mixture of bedding and manure) or in a deep pit below a slatted floor.  Previous research measured particulate matter, ammonia and other gases in bedded pack barn systems. Deep pit manure storages are expected to have different aerial nutrient losses and manure value compared to solid manure storage and handling. Few studies have looked at concentrations at animal level or aerial/temperature distributions in the animal zone. There is little to no documentation of the air quality impacts of long-term deep pit manure storage in naturally ventilated finishing cattle barns. The objective of this work is to describe the seasonal and spatial variations in aerial ammonia concentrations in deep pit beef cattle barns.

What Did We Do?

We measured ammonia concentrations among four pens in three beef cattle barns oriented east and west with deep pit manure storage during summer and fall conditions in Minnesota. We measured the concentration below the slatted floor (above the manure surface), 4-6 inches above the floor (floor level) and 4 ft above the floor (nose level). While collecting samples from within a pen, we also collected samples from the north and south wall openings surrounding the pen. We collected air and surface temperatures, air speed at cow level, and surface manure samples to supplement the concentration data. We collected measurements three times between 09:00 and 17:00 on sampling days. The cattle (if present) remained in the pen during measurement collection.

All farms had 12 ft deep pits below slatted floors, and pen stocking densities of 22 ft2 per head at capacity. Barn F finished beef cattle breeds under a monoslope roof, in four pens, with feed alleys on north and south side of pens. Two pens shared a common deep pit, and the farm pumped manure from the deep pits 1 week prior to the fall sampling period. Two pens were empty and the other two pens partially filled with cattle during the fall sampling period. Barn H finished dairy steers under a gable roof in a double-wide barn, in twelve pens over a deep pit and two pens with bedded packs, with a feed alley down the center of the barn. Four (east end) and eight (west end) pens shared common deep pits; the bedded pack pens were in the middle of the barn. The farm moved approximately 1 foot of manure from the east end pit to the west end pit one week prior to fall sampling period. Barn R finished dairy steers under a gable roof with four pens and a feed alley on the north side of the pens. All pens shared a common deep pit. Two pens were empty of cattle during the summer and fall sampling periods.

What Have We Learned?

The ammonia concentration levels differed based on the location in the pen area (Figures 1 and 2). As expected, the ammonia concentrations in the pit headspace above the manure surface was the greatest, and at times more than 10x the concentration at floor and nose level. The higher concentration levels measured at Barn F coincided with higher manure nitrogen levels (Total N and Ammonium-N) (Figure 2). Based on July and September measurements, higher ammonia concentration levels also coincided with higher ambient temperatures (Figure 1). The presence and size of cattle in the pens we measured did not strongly influence ammonia concentrations at any measurement height within a barn on sampling days.

Ammonia concentration is variable between barns, and within barns. However, at animal and worker level, average concentrations for the sampling periods were less than 10 ppm during the summer and fall periods. Higher gas levels can develop in the confined space below the slatted floor.

Future Plans

The air exchange between the deep pit headspace and room volume relates these two areas, but is challenging to measure. We are looking at indirect air exchange estimations using ammonia and other gas concentration measurements collected to quantify the amount of air movement through the slatted floor related to environmental conditions. Additional gas and environmental data collected will enhance our understanding of deep pit beef cattle barn environments.

Authors

Erin Cortus, Assistant Professor and Extension Engineer, University of Minnesota

ecortus@umn.edu

Brian Hetchler, Research Technician, University of Minnesota; Mindy Spiehs, Research Scientist, USDA-ARS; Warren Rusche, Extension Associate, South Dakota State University

Additional Information

USDA is an equal opportunity provider and employer

Acknowledgements

The research was supported through USDA NIFA Award No. 2015-67020-23453. We appreciate the producers’ cooperation for on-farm data collection. Thank you to S. Niraula and C. Modderman for assisting with measurements.

Figure 1. Average ammonia concentration levels in the animal and worker zone for three deep pit beef cattle barns during spring and fall sampling days, and the corresponding airspeed and temperature at cow nose level.
Figure 1. Average ammonia concentration levels in the animal and worker zone for three deep pit beef cattle barns during spring and fall sampling days, and the corresponding airspeed and temperature at cow nose level.
Figure 2. Average ammonia concentration levels at nose and manure surface levels for three deep pit beef cattle barns during spring and fall sampling days, and the corresponding surface* manure characteristics. (* Barn F 14-Sep-18 manure sample was an agitated sample collected during manure removal).
Figure 2. Average ammonia concentration levels at nose and manure surface levels for three deep pit beef cattle barns during spring and fall sampling days, and the corresponding surface* manure characteristics. (* Barn F 14-Sep-18 manure sample was an agitated sample collected during manure removal).

The authors are solely responsible for the content of these proceedings. The technical information does not necessarily reflect the official position of the sponsoring agencies or institutions represented by planning committee members, and inclusion and distribution herein does not constitute an endorsement of views expressed by the same. Printed materials included herein are not refereed publications. Citations should appear as follows. EXAMPLE: Authors. 2019. Title of presentation. Waste to Worth. Minneapolis, MN. April 22-26, 2019. URL of this page. Accessed on: today’s date.

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Sodium Bisulfate Treatment of Horse Stalls in the Southeastern United States

For the equine industry, concerns about ammonia (NH3) levels in the barn environment are multifaceted and include issues of animal welfare, animal and human health, and environmental impacts. In Florida, many performance horses are housed in stalls at least part of the day as are horses with allergic skin conditions and/or pasture associated asthma. The warm and humid climate produces favorable conditions for ammonia generation and fly emergence. Previous research has demonstrated the effectiveness of sodium bisulfate in lowering floor substrate and bedding pH, reducing ammonia concentrations, and fly populations in livestock facilities (poultry houses and dairies)1,2. However, research on application of sodium bisulfate in equine facilities is limited to two studies conducted in the northeastern United States3.

What did we do?

The objective of this pilot study was to determine the effects of sodium bisulfate (PLT®) application in a north central Florida equine facility on bedding pH, NH3 concentration, and fly counts. Four 12 x 12 ft stalls in a 20-stall barn were used, 2 control (CON) and 2 treated with sodium bisulfate (SB), individually housing mature geldings. Data were collected during the third week of August, 2018. Stalls were initially bedded with 67 lbs of wood shavings. Amount of product initially added to SB stalls was 14 lbs (manufacturer recommended application rate of 100 lbs/1,000 sqft) followed by 7 lbs daily for 4 days. Horses were housed in stalls overnight (12 hours/day) and stalls cleaned (manure and wet bedding removed) once/day. An aspirating pump and gas detection tubes (Kitagawa, Japan) were used to determine NH3 concentration before stall cleaning (AM measurement), to allow for manure and urine accumulation, and 10 hours post stall cleaning (PM measurement). Three 5-gallon buckets were placed over the stall surface in a triangular pattern to standardize airflow and the location of each bucket was marked to allow replication across AM and PM readings. OnSet HOBO loggers were used to monitor temperature and relative humidity. Fly traps containing no fly attractant, were suspended 8 feet above the floor in the center of each stall to determine fly counts.

What Have We Learned?

Background (cleaned stalls without bedding material; rubber mats only) and baseline (bedded stalls) NH3 concentrations were < 5 ppm and not different between SB and CON stalls. NH3 concentrations had a cumulative effect and were greater on day 3 (69.8 ppm) compared to day 1 (< 5 ppm) and day 2 (16.7 ppm). NH3 concentrations were greater in CON stalls (28.6 ppm) compared to SB stalls (< 5 ppm). Bedding pH was lower in SB stalls (1.82) compared to CON stalls (6.16) demonstrating an overall treatment effect, but pH of the bedding increased over the duration of the study. The number of flies caught in traps did not differ between treatments, although fly counts did increase over time. Reductions in pH and NH3 observed in the present study were comparable to previous studies. We expected reductions in flies in stalls treated with SB, however, fly counts were extremely low overall and a different approach for quantifying fly numbers may be necessary.

Future Plans

Future research directions include testing different application rates for equine stalls and determining efficacy of SB with different bedding types. Additional studies to investigate the effectiveness of SB in mitigating NH3 emissions in equine facilities4 and reducing fly populations and bacteria in stalls should be pursued. There is also potential to assess the benefits of SB application near manure storage areas on equine operations.

Corresponding author, title, affiliation and email

Carissa Wickens, Extension Equine Specialist, University of Florida.   cwickens@ufl.edu

Other authors: Jill Bobel, Biological Scientist, University of Florida; Danielle Collins, Graduate Student, University of Florida; Alex Basso, Graduate Student, University of Florida

Additional information:

1Johnson, T. M. and B. Murphy. 2008. Use of sodium bisulfate to reduce ammonia emissions from poultry and livestock housing. Proceedings of the Mitigating Air Emissions from Animal Feeding Operations Conference, Des Moines, IA. Iowa State University, pp. 74-78.

2Sun, H., Y. Pan, Y. Zhao, W. A. Jackson, L. M. Nuckles, I. L. Malkina, V. E. Arteaga and F. M. Mitloehner. 2008. Effects of sodium bisulfate on alcohol, amine, and ammonia emissions from dairy slurry. J. Environmental Quality 37:608-614. 

3Sweeney, C.R., S.M. McDonnell, G.E. Russell, and M. Terzich. 1997. Effect of sodium bisulfate on ammonia concentration, fly population, and manure pH in a horse barn. Am. J. Vet. Res. 57(12):1795-1798.

4Weir, J., H. Li, L.K. Warren, E. Macon, C. Wickens. 2017. Evaluating the impact of ammonia emissions from equine operations on the environment. Waste to Worth: Spreading Science and Solutions. Cary, NC. April 18-21, 2017. https://lpelc.org/evaluating-the-impact-of-ammonia-emissions-from-equine-operations-on-the-environment/. Accessed on: February 28, 2019.

Additional information regarding this project is available by contacting Carissa Wickens (cwickens@ufl.edu), or Jill Bobel (jbrides2@ufl.edu).

Acknowledgements:

The authors wish to thank Dr. Josh Payne, Technical Services Manager, Jones-Hamilton Company, Agricultural Division, and Dr. Hong Li, Associate Professor, Department of Animal and Food Sciences, University of Delaware for providing technical expertise and support for this project. We would also like to thank Carol Vasco, Tayler Hansen, Agustin Francisco, and Claudia Lopez for their assistance with data collection.

Figure 1: Placement of buckets over the stall floor for measurement of NH3 concentrations. The ammonia pump with attached gas detection tube was placed through a small hole drilled into the top of each bucket.
Figure 1: Placement of buckets over the stall floor for measurement of NH3 concentrations. The ammonia pump with attached gas detection tube was placed through a small hole drilled into the top of each bucket.
Figure 2: Average daytime and nighttime temperatures and percent relative humidity during the study period.
Figure 2: Average daytime and nighttime temperatures and percent relative humidity during the study period.

The authors are solely responsible for the content of these proceedings. The technical information does not necessarily reflect the official position of the sponsoring agencies or institutions represented by planning committee members, and inclusion and distribution herein does not constitute an endorsement of views expressed by the same. Printed materials included herein are not refereed publications. Citations should appear as follows. EXAMPLE: Authors. 2019. Title of presentation. Waste to Worth. Minneapolis, MN. April 22-26, 2019. URL of this page. Accessed on: today’s date.

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