Antimicrobial resistance is a complex issue as it is comprised of not only pathogenic bacteria, but also non-pathogens which share genes within complex environmental systems, such as agricultural fields. This webinar describes potential measures to reduce pathogen and antimicrobial resistance in manure as well as potential fate and transport of manure pathogens and antimicrobial resistance following land application of manure. This presentation was originally broadcast on May 17, 2019. More… Continue reading “Reduction and fate of manure pathogens and antimicrobial resistance”
The main purpose of this research project is an evaluation of the current products available in the open marketplace for using in deep pit swine manure structure as to their effectiveness in mitigation of odors and reduction of hydrogen sulfide (H2S), ammonia (NH3), 11 odorous volatile organic compounds (VOCs) and greenhouse gas (CO2, methane and nitrous oxide) emissions from stored swine manure. At the end of each trial, hydrogen sulfide and ammonia concentrations are measured during and immediately after the manure agitation process to simulate pump-out conditions. In addition, pit manure additives are tested for their impact on manure properties including solids content and microbial community.
What Did We Do?
We are using 15 reactors simulating swine manure storage (Figure 1) filled with fresh swine manure (outsourced from 3 different farms) to test simultaneously four manure additive products using manufacturer recommended dose for each product. Each product is tested in 3 identical dosages and storage conditions. The testing period starts on Day 0 (application of product following the recommended dosage by manufacturer) with weekly additions of manure from the same type of farm. The headspace ventilation of manure storage is identical and controlled to match pit manure storage conditions. Gas and odor samples from manure headspace are collected weekly. Hydrogen sulfide and ammonia concentrations are measured in real time with portable meters (both are calibrated with high precision standard gases). Headspace samples for greenhouse gases are collected with a syringe and vials, and analyzed with a gas chromatograph calibrated for CO2, methane and nitrous oxide. Volatile organic compounds are collected with solid-phase microextraction probes and analyzed with a gas chromatography-mass spectrometry (Atmospheric Environment 150 (2017) 313-321). Odor samples are collected in 10 L Tedlar bags and analyzed using the olfactometer with triangular forced-choice method (Chemosphere, 221 (2019) 787-783). To agitate the manure for pump-out simulation, top and bottom ‘Manure Sampling Ports’ (Figure 1) are connected to a liquid pump and cycling for 5 min. Manure samples are collected at the start and end of the trial and are analyzed for nitrogen content and bacterial populations.
The effectiveness of the product efficacy to mitigate emissions is estimated by comparing gas and odor emissions from the treated and untreated manure (control). The mixed linear model is used to analyze the data for statistical significance.
What we have learned?
U.S. pork industry will have science-based, objectively tested information on odor and gas mitigation products. The industry does not need to waste precious resources on products with unproven or questionable performance record. This work addresses the question of odor emissions holistically by focusing on what changes that are occurring over time in the odor/odorants being emitted and how does the tested additive alter manure properties including the microbial community. Additionally, we tested the hydrogen sulfide and ammonia emissions during the agitation process simulating pump-out conditions. For both gases, the emissions increased significantly as shown in Figure 2. The Midwest is an ideal location for swine production facilities as the large expanse of crop production requires large fertilizer inputs, which allows manure to be valued as a fertilizer and recycled and used to support crop production.
We develop and test sustainable technologies for mitigation of odor and gaseous emissions from livestock operations. This involves lab-, pilot-, and farm-scale testing. We are pursuing advanced oxidation (UV light, ozone, plant-based peroxidase) and biochar-based technologies.
Baitong Chen, M.S. student, Iowa State University
Jacek A. Koziel*, Prof., Iowa State University (firstname.lastname@example.org)
Daniel S. Andersen, Assoc. Prof., Iowa State University
David B. Parker, Ph.D., P.E., USDA-ARS-Bushland
- Heber et al., Laboratory Testing of Commercial Manure Additives for Swine Odor Control. 2001.
- Lemay, S., Stinson, R., Chenard, L., and Barber, M. Comparative Effectiveness of Five Manure Pit Additives. Prairie Swine Centre and the University of Saskatchewan.
- 2017 update – Air Quality Laboratory & Olfactometry Laboratory Equipment – Koziel’s Lab. doi: 10.13140/RG.2.2.29681.99688.
- Maurer, D., J.A. Koziel. 2019. On-farm pilot-scale testing of black ultraviolet light and photocatalytic coating for mitigation of odor, odorous VOCs, and greenhouse gases. Chemosphere, 221, 778-784; doi: 10.1016/j.chemosphere.2019.01.086.
- Maurer, D.L, A. Bragdon, B. Short, H.K. Ahn, J.A. Koziel. 2018. Improving environmental odor measurements: comparison of lab-based standard method and portable odour measurement technology. Archives of Environmental Protection, 44(2), 100-107. doi: 10.24425/119699.
- Maurer, D., J.A. Koziel, K. Bruning, D.B. Parker. 2017. Farm-scale testing of soybean peroxidase and calcium peroxide for surficial swine manure treatment and mitigation of odorous VOCs, ammonia, hydrogen sulfide emissions. Atmospheric Environment, 166, 467-478. doi: 10.1016/j.atmosenv.2017.07.048.
- Maurer, D., J.A. Koziel, J.D. Harmon, S.J. Hoff, A.M. Rieck-Hinz, D.S Andersen. 2016. Summary of performance data for technologies to control gaseous, odor, and particulate emissions from livestock operations: Air Management Practices Assessment Tool (AMPAT). Data in Brief, 7, 1413-1429. doi: 10.1016/j.dib.2016.03.070.
We are thankful to (1) National Pork Board and Indiana Pork for funding this project (NBP-17-158), (2) cooperating farms for donating swine manure and (3) manufacturers for providing products for testing. We are also thankful to coworkers in Dr. Koziel’s Olfactometry Laboratory and Air Quality Laboratory, especially Dr. Chumki Banik, Hantian Ma, Zhanibek Meiirkhanuly, Lizbeth Plaza-Torres, Jisoo Wi, Myeongseong Lee, Lance Bormann, and Prof. Andrzej Bialowiec.
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