Tag: nitrous oxide

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Emission of ammonia, hydrogen sulfide, and greenhouse gases following application of aluminum sulfate to beef feedlot surfaces

Purpose

Alum has been successfully used in the poultry industry to lower ammonia (NH3) emission from the barns. However, it has not been evaluated to reduce NH3 on beef feedlot surfaces. Additionally, it is not known how it would affect other common emissions from beef feedlot surfaces. The purpose of this study was to determine the effect of adding aluminum sulfate to beef feedlot surfaces on NH3, hydrogen sulfide (H2S), carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O) emissions.

What Did We Do?

Eight feedlot pens (30 animals per pen) at the U.S. Meat Animal Research Center feedlot were utilized. The pens had a central mound constructed on manure and soil and 3 m concrete apron by the feed bunk and cattle were fed a corn-silage based diet. Four pens (30 cattle/pen) had 10% (g g-1) alum applied to the 6 meters immediately behind the concrete bunk apron and four did not receive alum. The amount of alum added to the area was determined on a mass basis for a depth of 5 cm of feedlot surface material (FSM) using the estimated density of feedlot surface material for Nebraska feedlots (1.5 g cm−3). On sampling days, six representative grab samples were collected from the feedlot surface from the six-meter area behind the bunk apron in each pen; samples were combined within pen to make three representative replicates per pen (N=24). Each of the three pooled samples per pen were measured for pH, NH3, H2S, CH4, CO2, and N2O using petri dishes and wind tunnels in an environmental chamber at an ambient temperature of 25°C (77°F) and 50% relative humidity. Flux measurements for NH3, H2S, CH4, CO2, and N2O flux were measured for 15 minutes using Thermo Fisher Scientific 17i, 450i, 55i, 410iQ, and 46i gas analysis instruments, respectively. Samples were analyzed at day -1, 0, 5, 7, 12, 14, 19, 21, and 26.

What Have We Learned?

Addition of alum lowered pH of FSM from 8.3 to 4.8 (p < 0.01) and the pH remained lower in alum-treated pens for 26 days (p < 0.01). Although the pH remained low, NH3 flux was only lower (p < 0.01) at day 0 and day 5 for alum-treated pens compared to the pens with no alum treatment. Nitrous oxide emission was not affected by alum treatment (6.2 vs 5.7 mg m-2 min-1, respectively for 0 and 10% alum treated pens). Carbon dioxide emission was lower for alum-treated pens than non-treated pens from day 5 until the end of the study (p < 0.05), perhaps due to suppressed microbial activity from the lower pH. Hydrogen sulfide emission was higher (p < 0.05) from alum-treated feedlot surface material (0.8 mg m-2 min-1) compared to non-treated feedlot surface material (0.3 mg m-2 min-1), likely due to addition of sulfate with alum. Methane emission was also higher in alum-treated pens (173.6 mg m-2 min-1) than non-treated pens (81.4 mg m-2 min-1). The limited reduction in NH3, along with increased H2S and CH4 emission from the FSM indicates that alum is not a suitable amendment to reduce emissions from beef feedlot surfaces.

Table 1. pH, ammonia (NH3), hydrogen sulfide (H2S), methane (CH4), carbon dioxide (CO2) and nitrous oxide (N2O) emission from feedlot surface material treated with 0 or 10% alum (g g-1 mass basis).
pH NH3
(mg m-2 min-1)		 H2S
(mg m-2 min-1)		 CH4
(mg m-2 min-1)		 CO2
(mg m-2 min-1)		 N2O
(mg m-2 min-1)
Day 0% Alum 10% Alum 0% Alum 10% Alum 0% Alum 10% Alum 0% Alum 10% Alum 0% Alum 10% Alum 0% Alum 10% Alum
-1 8.1 8.3 229.6d 515.9c 0.3 0.4 136.3 x 73.4w 4,542 3,234 3.1 4.2
0 8.3a 4.8b 163.0c 32.4d 0.2 f 1.8 e 43.1 x 193.8w 4,372 5,294 2.9 1.8
5 8.5a 6.3b 279.5c 83.6d 0.4 0.5 84.1 x 309.5w 404y 1,347z 6.0 6.8
7 8.6a 6.7b 120.2 130.0 0.6 f 1.2e 53.4 61.7 468 y 1,903z 15.3 10.9
12 8.6a 7.2b 418.0 320.3 0.3 0.3 104.5 145.7 3,742y 1,939z 3.3 8.0
14 8.9a 7.6b 229.0 145.5 0.2 0.4 25.4x 180.7w 4,203y 2,018z 11.5 9.3
19 8.6a 7.5b 228.0 225.1 0.1 f 1.1e 132.3x 254.7w 5,999y 3,116z 6.9 5.8
21 8.4a 7.2b 232.0 257.0 0.5 0.8 81.9x 250.0w 4,324y 2,477z 2.2 1.9
26 8.6a 8.0b 584.5c 319.9d 0.1f 0.7e 72.2 92.9 5,534y 3,540z 4.7 2.9
Within a parameter and day, different superscripts indicate a significant difference (p < 0.05) between the emissions from the feedlot surface material treated with 0% and 10% alum.

Future Plans

Future research will evaluate the use of aluminum chloride instead of aluminum sulfate to lower pH of FSM and retain nitrogen. Additionally, microbial amendments are being evaluated to determine if they can reduce gaseous emissions from the feedlot surface.

Authors

Presenting author

Mindy J. Spiehs, Research Animal Scientists, USDA ARS Meat Animal Research Center

Corresponding author

Bryan L. Woodbury, Agricultural Engineer, USDA ARS Meat Animal Research Center

Corresponding author email address

bryan.woodbury@usda.gov

Additional Information

For additional information about the use of alum as a feedlot surface amendment, readers are direct to the following: Effects of using aluminum sulfate (alum) as a surface amendment in beef cattle feedlots on ammonia and sulfide emissions. 2022. Sustainability 14(4): 1984 – 2004. https://doi.org/10.3390/su14041984

Acknowledgements

The authors wish to acknowledge USMARC technicians Alan Kruger and Jessie Clark for their assistance with data collection and analysis.

 

The authors are solely responsible for the content of these proceedings. The technical information does not necessarily reflect the official position of the sponsoring agencies or institutions represented by planning committee members, and inclusion and distribution herein does not constitute an endorsement of views expressed by the same. Printed materials included herein are not refereed publications. Citations should appear as follows. EXAMPLE: Authors. 2022. Title of presentation. Waste to Worth. Oregon, OH. April 18-22, 2022. URL of this page. Accessed on: today’s date.

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How are Filth Flies Involved in Wasting Nitrogen?

Purpose

Filth flies are species from the Diptera order associated with animal feces and decomposing waste. Beef cattle raised on open pastures are especially susceptible to two species of filth flies: Face flies (Musca autumnalis De Geer) and Horn flies (Haematobia irritans (L.))  because these flies develop exclusively in fresh cattle manure. Filth fly impact on cattle health is related not only to the loss of body weight but also to the transmission of diseases like pink eye and mastitis (Basiel, 2020; Campbell, 1976; Hall, 1984; Nickerson et al., 1995).

Nitrogen losses from cattle’s manure has been reported for domestic flies (Musca domestica) and bottle flies (Neomyia cornicina) (Iwasa et al., 2015; Macqueen & Beirne, 1975). Despite the regular presence of face fly and horn fly in pastures, their effect on the nutrient cycles is little known. The purpose of this study is to understand the relationship between filth fly’s presence in cattle manure with the nitrogen losses caused by an increase in ammonia and nitrous oxide emissions.

What Did We Do?

The study was conducted in four pastures in the Georgia Piedmont: two near Watkinsville and two near Eatonton during June, July, and August of 2021. Ammonia volatilization and nitrous oxide emissions were measured on days 1, 4, 8, and 15 following dung deposition. Manure samples were collected on days 1 and 15. A static chamber was sealed for 24 h on each sampling date to capture manure’s ammonia and nitrous oxide emissions. In each chamber, a glass jar with boric acid was used to trap ammonia, and gas samples were collected. The gas samples were analyzed for nitrous oxide with a Varian Star 3600 CX Gas Chromatograph using an electron capture detector.

The number of filth flies was determined using a net trap covered by a black cloth that was set after 1 min of manure deposition, allowing the flies to oviposit for 10 min. On the days in which ammonia was not measured, a net trap was set to avoid additional oviposits, and record the emergence of filth flies. On the 15th day, we collected the filth flies that emerged from the eggs deposited in the manure during the first day.

What Have We Learned?

We found that cattle’s manure nitrogen loss as nitrous oxide (N2O) and ammonia (NH3) emissions have a direct relationship with the number of horn flies and face flies in the dung, Figure 1. Eighty percent of the flies trapped were horn flies. Dung with less than 5 flies can emit as little as 0.11 mg of N/kg of manure per day, while cattle manure with more than 30 flies can increase this emission by more than 10 times.

Figure 1 Nitrogen emissions such as nitrous oxide and ammonia (mg/kg of manure) and number of filth flies.

Every extra filth fly in manure can increase N emissions by 0.03 mg per kg of manure per day. According to NRCS, 59.1 lbs. of fresh manure is produced by a cow (approx. 1 000 pounds animal) every day (NRCS, 1995). Considering an average of 85 % relative humidity, 4.03 kg of dry manure can be produced per cow day. The actual economic threshold for horn fly is 200 flies per animal (Hogsette et al., 1991; Schreiber et al., 1987), considering a 1 to 1 sex ratio during emergence (Macqueen & Doube, 1988) we are assuming 100 female flies. Since the capacity of horn flies is 8-13 eggs per day (Lysyk, 1999), 100 female horn flies can generate approximately 1,000 new flies every day.  Calculating the nitrogen emissions (4.03 kg of dry manure X 0.03 mg N kg manure x 1,000 flies per day) results in 121 mg of N loss per cow per day when assuming the number of flies is just at the economic threshold. In January of 2022, USDA released the Southern Region Cattle Inventory with a total of 91.9 million head, from which 30.1 million were beef cows (USDA, 2022). Considering the earlier numbers, the horn fly presence in the beef cattle of the Southern Region could be emitting 3,639 kg of Nitrogen to the atmosphere every day.

Future Plans

We will continue the study on ammonia and nitrous oxide emissions under the same conditions during another year to confirm the trends and accuracy of the results. Also, we will implement a study to analyze the effect of the introduction of a parasitic wasp Spalangia endius as a biological control on horn fly and face fly populations and therefore on the manure’s nitrogen losses.

Authors

Presenting author

Natalia B. Espinoza, Research Assistant, Department of Crop and Soil Science, University of Georgia

Corresponding author

Dr. Dorcas H. Franklin, Professor, Department of Crop and Soil Sciences, University of Georgia

Corresponding author email address

dfrankln@uga.edu or dory.franklin@uga.edu

Additional authors

Anish Subedi, Research Assistant, Department of Crop and Soil Science, University of Georgia

Dr. Miguel Cabrera, Professor, Department of Crop and Soil Sciences, University of Georgia

Dr. Nancy Hinkle, Professor, Department of Entomology, University of Georgia

Dr. S. Lawton Stewart, Professor, Department of Animal and Dairy Science, University of Georgia

Additional Information

Basiel, B. (2020). Genomic Evaluation of Horn Fly Resistance and Phenotypes of Cholesterol Deficiency Carriers in Holstein Cattle [PennState University]. Electronic Theses and Dissertations for Graduate Students.

Campbell, J. B. (1976). Effect of Horn Fly Control on Cows as Expressed by Increased Weaning Weights of Calves. Journal of Economic Entomology, 69(6), 711-712. https://doi.org/DOI 10.1093/jee/69.6.711

Hall, R. D. (1984). Relationship of the Face Fly (Diptera, Muscidae) to Pinkeye in Cattle – a Review and Synthesis of the Relevant Literature. Journal of Medical Entomology, 21(4), 361-365. https://doi.org/DOI 10.1093/jmedent/21.4.361

Hogsette, J. A., Prichard, D. L., & Ruff, J. P. (1991). Economic-Effects of Horn Fly (Diptera, Muscidae) Populations on Beef-Cattle Exposed to 3 Pesticide Treatment Regimes. Journal of Economic Entomology, 84(4), 1270-1274. https://doi.org/DOI 10.1093/jee/84.4.1270

Iwasa, M., Moki, Y., & Takahashi, J. (2015). Effects of the Activity of Coprophagous Insects on Greenhouse Gas Emissions from Cattle Dung Pats and Changes in Amounts of Nitrogen, Carbon, and Energy. Environmental Entomology, 44(1), 106-113. https://doi.org/10.1093/ee/nvu023

Lysyk, T. J. (1999). Effect of temperature on time to eclosion and spring emergence of postdiapausing horn flies (Diptera : Muscidae). Environmental Entomology, 28(3), 387-397. https://doi.org/DOI 10.1093/ee/28.3.387

Macqueen, A., & Beirne, B. P. (1975). Influence of Some Dipterous Larvae on Nitrogen Loss from Cattle Dung. Environmental Entomology, 4(6), 868-870. https://doi.org/DOI 10.1093/ee/4.6.868

Macqueen, A., & Doube, B. M. (1988). Emergence, Host-Finding and Longevity of Adult Haematobia-Irritans-Exigua Demeijere (Diptera, Muscidae). Journal of the Australian Entomological Society, 27, 167-174. <Go to ISI>://WOS:A1988P906100002

Nickerson, S. C., Owens, W. E., & Boddie, R. L. (1995). Symposium – Mastitis in Dairy Heifers – Mastitis in Dairy Heifers – Initial Studies on Prevalence and Control. Journal of Dairy Science, 78(7), 1607-1618. https://doi.org/DOI 10.3168/jds.S0022-0302(95)76785-6

NRCS, N. R. C. S. (1995). Animal Manure Management. RCA Publication Archive(7). https://www.nrcs.usda.gov/wps/portal/nrcs/detail/null/?cid=nrcs143_014211

Schreiber, E. T., Campbell, J. B., Kunz, S. E., Clanton, D. C., & Hudson, D. B. (1987). Effects of Horn Fly (Diptera, Muscidae) Control on Cows and Gastrointestinal Worm (Nematode, Trichostrongylidae) Treatment for Calves on Cow and Calf Weight Gains. Journal of Economic Entomology, 80(2), 451-454. https://doi.org/DOI 10.1093/jee/80.2.451

USDA. (2022). Southern Region News Release Cattle Inventory. https://www.nass.usda.gov/Statistics_by_State/Regional_Office/Southern/includes/Publications/Livestock_Releases/Cattle_Inventory/Cattle2022.pdf

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Production of Greenhouse Gases, Ammonia, Hydrogen Sulfide, and Odorous Volatile Organic Compounds from Manure of Beef Feedlot Cattle Implanted with Anabolic Steroids

Animal production is part of a larger agricultural nutrient recycling system that includes soil, water, plants, animals and livestock excreta. When inefficient storage or utilization of nutrients occurs, parts of this cycle become overloaded. The U.S. Beef industry has made great strides in improving production efficiency with a significant emphasis on improving feed efficiency. Improved feed efficiency results in fewer excreted nutrients and volatile organic compounds (VOC) that impair environmental quality. Anabolic steroids are used to improve nutrient feed efficiency which increases nitrogen retention and reduces nitrogen excretion. This study was conducted to determine the methane (CH4), carbon dioxide (CO2), nitrous oxide (N2O), odorous VOCs, ammonia (NH3), and hydrogen sulfide (H2S) production from beef cattle manure and urine when aggressive steroid implants strategies were used instead of moderate implant strategies.

What Did We Do?

Two groups of beef steers (60 animals per group) were implanted using two levels of implants (moderate or aggressive). This was replicated three times, twice with spring-born calves and once with fall-born calves, for a total of 360 animals used during the study. Both moderate and aggressive treatment groups received the same initial implant that contain 80 mg trenbolone acetate and 16 mg estradiol. At second implant, steers in the moderate group received an implant that contained 120 mg trenbolone acetate and 24 mg estradiol, while those in the aggressive group received an implant that contained 200 mg trenbolone acetate and 20 mg estradiol. Urine and feces samples were collected individually from 60 animals that received a moderate implant and 60 animals that received an aggressive implant at each of three sampling dates (Spring and Fall 2017 and Spring 2018). Within each treatment, fresh urine and feces from five animals were mixed together to make a composite sample slurry (2:1 ratio of manure:urine) and placed in a petri dish. There were seven composite mixtures for each treatment at each sampling date. Wind tunnels were used to pull air over the petri dishes. Ammonia, carbon dioxide, and nitrous oxide concentrations were measured using an Innova 1412 Photoacoustic Gas Analyzer. Hydrogen sulfide and methane were measured using a Thermo Fisher Scientific 450i and 55i, respectively. Gas measurements were taken a minimum of six times over 24- to 27-day sampling periods.

What Have We Learned?

Flux of ammonia, hydrogen sulfide, methane, nitrous oxide, and total aromatic volatile organic compounds were significantly lower when an aggressive implant strategy was used compared to a moderate implant strategy. However, the flux of total branched-chained volatile organic compounds from the manure increased when aggressive implants were used compared to moderate implants. Overall, this study suggests that air quality may be improved when an aggressive implant is used in beef feedlot animals.

Table 1. Overall average flux of compounds from manure (urine + feces) from beef feedlot cattle implanted with a moderatea or aggressiveb anabolic steroid.
Hydrogen Sulfide Ammonia Methane Carbon Dioxide Nitrous  Oxide Total Sulfidesc Total SCFAd Total BCFAe Total Aromaticsf
µg m-2 min-1 ——–mg m-2 min-1——–
Moderate 4.0±0.1 2489.7±53.0 117.9±4.0 8795±138 8.6±0.1 0.7±0.1 65.2±6.6 5.9±0.5 2.9±0.3
Aggressive 2.7±0.2 2186.4±46.2 104.0±3.8 8055±101 7.4±0.1 0.8±0.1 63.4±5.7 7.6±0.8 2.1±0.2
P-value 0.01 0.04 0.01 0.01 0.01 0.47 0.83 0.05 0.04
aModerate treatment =  120 mg trenbolone acetate and 24 mg estradiol at second implant; bAggressive treatment = 200 mg trenbolone acetate and 20 mg estradiol at second implant; cTotal sulfides = dimethyldisulfide and dimethyltrisulfide; dTotal straight-chained fatty acids (SCFA) = acetic acid, propionic acid, butyric acid, valeric acid, hexanoic acid, and heptanoic acid;  eTotal branch-chained fatty acids (BCFA) = isobutyric acid and isovaleric acid; fTotal aromatics = phenol, 4-methylphenol, 4-ethylphenol, indole, and skatole

Future Plans
Urine and fecal samples are being evaluated to determine the concentration of steroid residues in the livestock waste and the nutrient content (nitrogen, phosphorus, potassium and sulfur) of the urine and feces.

Authors

mindy.spiehs@ars.usda.gov Mindy J. Spiehs, Research Animal Scientist, USDA ARS Meat Animal Research Center, Clay Center, NE

Bryan L. Woodbury, Agricultural Engineer, USDA ARS Meat Animal Research Center, Clay Center, NE

Kristin E. Hales, Research Animal Scientist, USDA ARS Meat Animal Research Center, Clay Center, NE

Additional Information

Will be included in Proceedings of the 2019 Annual International Meeting of the American Society of Agricultural and Biological Engineers.

USDA is an equal opportunity provider and employer. 

Acknowledgements

The authors wish to thank Alan Kruger, Todd Boman, Bobbi Stromer, Brooke Compton, John Holman, Troy Gramke and the USMARC Cattle Operations Crew for assistance with data collection.

The authors are solely responsible for the content of these proceedings. The technical information does not necessarily reflect the official position of the sponsoring agencies or institutions represented by planning committee members, and inclusion and distribution herein does not constitute an endorsement of views expressed by the same. Printed materials included herein are not refereed publications. Citations should appear as follows. EXAMPLE: Authors. 2019. Title of presentation. Waste to Worth. Minneapolis, MN. April 22-26, 2019. URL of this page. Accessed on: today’s date.

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Production of Greenhouse Gases and Odorous Compounds from Manure of Beef Feedlot Cattle Fed Diets With and Without Ionophores

Ionophores are a type of antibiotics that are used in cattle production to shift ruminal fermentation patterns. They do not kill bacteria, but inhibit their ability to function and reproduce. In the cattle rumen, acetate, propionate, and butyrate are the primary volatile fatty acids produced. It is more energetically efficient for the rumen bacteria to produce acetate and use methane as a hydrogen sink rather than propionate. Ionophores inhibit archaea forcing bacteria to produce propionate and butyrate as hydrogen sinks rather than working symbiotically with methanogens to produce methane as a hydrogen sink. Numerous research studies have demonstrated performance advantages when ionophores are fed to beef cattle, but few have considered potential environmental benefits of feeding ionophores. This study was conducted to determine if concentrations of greenhouse gases, odorous volatile organic compounds (VOC), ammonia, and hydrogen sulfide from beef cattle manure could be reduced when an ionophore was fed to finishing cattle.

What Did We Do?

Four pens of feedlot cattle were fed an ionophore (monensin) and four pens received no ionophore (n=30 animals/pen). Samples were collected six times over a two-month period. A minimum of 20 fresh fecal pads were collected from each feedlot pen at each collection. Samples were mixed within pen and a sub-sample was placed in a small wind-tunnel. Duplicate samples for each pen were analyzed. Ammonia, carbon dioxide (CO2), and nitrous oxide (N2O) concentrations were measured using an Innova 1412 Photoacoustic Gas Analyzer. Hydrogen sulfide (H2S) and methane (CH4) were measured using a Thermo Fisher Scientific 450i and 55i, respectively.

What Have We Learned?

 

Table 1. Overall average concentration of compounds from feces of beef feedlot cattle fed diets with and without monensin.
Hydrogen Sulfide Ammonia Methane Carbon Dioxide Nitrous  Oxide Total Sulfidesa Total  SCFAb Total BCFAc Total Aromaticsd
µg L-1 —————-mg L-1—————-
No Monensin 87.3±2.2 1.0±0.2 4.3±0.1 562.5±2.2 0.4±0.0 233.4±18.3 421.6±81.9 16.8±3.1 83.7±6.4
Monensin 73.9±1.4 1.1±0.2 3.2±0.2 567.1±2.1 0.5±0.0 145.5±10.9 388.9±32.5 20.3±2.3 86.4±5.6
P-value 0.30 0.40 0.01 0.65 0.21 0.01 0.79 0.48 0.75
aTotal sulfides = dimethyldisulfide and dimethyltrisulfide; bTotal straight-chained fatty acids (SCFA) = acetic acid, propionic acid, butyric acid, valeric acid, hexanoic acid, and heptanoic acid;  cTotal branch-chained fatty acids (BCFA) = isobutyric acid and isovaleric acid; dTotal aromatics = phenol, 4-methylphenol, 4-ethylphenol, indole, and skatole

Total CH4 concentration decreased when monensin was fed. Of the VOCs measured, only total sulfide concentration was lower for the manure from cattle fed monensin compared to those not fed monensin. Ammonia, N2O, CO2, H2S, and all other odorous VOC were similar between the cattle fed monensin and those not fed monensin. The results only account for concentration of gases emitted from the manure and do not take into account any urinary contributions, but indicate little reduction in odors and greenhouse gases when monensin was fed to beef finishing cattle.

Future Plans

A study is planned for April – July 2019 to measure odor and gas emissions from manure (urine and feces mixture) from cattle fed with and without monensin. Measurements will also be collected from the feedlot surface of pens with cattle fed with and without monensin.  

Authors

Mindy J. Spiehs, Research Animal Scientist, USDA ARS Meat Animal Research Center, Clay Center, NE

mindy.spiehs@ars.usda.gov

Bryan L. Woodbury, Agricultural Engineer, USDA ARS Meat Animal Research Center, Clay Center, NE

Kristin E. Hales, Research Animal Scientist, USDA ARS Meat Animal Research Center, Clay Center, NE

Additional Information

Dr. Hales also looked at growth performance and E. coli shedding when ionophores were fed to finishing beef cattle. This work is published in Journal of Animal Science.

Hales, K.E., Wells, J., Berry, E.D., Kalchayanand, N., Bono, J.L., Kim, M.S. 2017. The effects of monensin in diets fed to finishing beef steers and heifers on growth performance and fecal shedding of Escherichia coli O157:H7. Journal of Animal Science. 95(8):3738-3744. https://pubmed.ncbi.nlm.nih.gov/28805884/.

USDA is an equal opportunity provider and employer.

Acknowledgements

The authors wish to thank Alan Kruger, Todd Boman, and the USMARC Cattle Operations Crew for assistance with data collection.

The authors are solely responsible for the content of these proceedings. The technical information does not necessarily reflect the official position of the sponsoring agencies or institutions represented by planning committee members, and inclusion and distribution herein does not constitute an endorsement of views expressed by the same. Printed materials included herein are not refereed publications. Citations should appear as follows. EXAMPLE: Authors. 2019. Title of presentation. Waste to Worth. Minneapolis, MN. April 22-26, 2019. URL of this page. Accessed on: today’s date.

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How Well Do We Understand Nitrous Oxide Emissions from Open-lot Cattle Systems?

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Purpose

Nitrous oxide (N2O) emissions from concentrated animal feeding operations, including cattle feedyards, have become an important research topic. However, there are limitations to current measurement techniques, uncertainty in the magnitude of feedyard N2O fluxes, and a lack of effective mitigation methods. There are uncertainties in the pathway of feedyard N2O production, the dynamics of nitrogen transformations in these manure-based systems, and how N2O emissions differ with changes in climate and feedyard management.

What Did We Do?

A literature review was conducted to assess the state-of-the-science of N2O production and emission from open-lot beef cattle feedyards and dairies. The objective was to assess N2O emission from cattle feedyards, including comparison of measured and modeled emission rates, discussion of measurement methods, and evaluation of mitigation options. In addition, laboratory, pilot-scale, and field-scale chamber studies were conducted to quantify and characterize N2O emissions from beef cattle manure. These studies led to new empirical model to predict feedyard N2O fluxes as a function of temperature and manure nitrate and water contents. Organic matter stability/availability was important in predicting manure-derived N2O emissions: inclusion of data for dissolved organic carbon content and Ultraviolet-visible (UV-vis) spectroscopic indices of molecular weight, complexity and degree humification improved model performance against measured data.

What Have We Learned?

Published annual per capita flux rates for beef cattle feedyards and open-lot dairies in arid climates were highly variable and ranged from 0.002 to 4.3 kg N2O animal-1 yr-1. On an area basis, published emission rates ranged from 0 to 41 mg N2O m-2 h-1. From these studies and the Intergovernmental Panel on Climate Change emission factors, calculated daily per capita N2O fluxes averaged 18 ± 10 g N2O animal-1 d-1 (range, 0.04–67 g N2O animal-1 d-1). Some of this variability is inherently derived from differences in manure management practices and animal diets among open-lot cattle systems. However, it was proposed that other major causes of variation were inconsistency in measurement techniques, and irregularity in N2O production due to environmental conditions.

For modeling studies, N2O emissions were measured during 15 chamber studies (10 chambers per study) on commercial Texas feedyards, where N2O emissions ranged from below detection to 101 mg N2O m-2 h-1. Numerous feedyard and manure data were collected and regression analyses were used to determine key variables involved in feedyard N2O losses. Based on these data, two models were developed: (1) a simple model that included temperature, manure water content, and manure nitrate concentration, and (2) a more complex model that included UV-vis spectral data that provided an estimate of organic matter stability. Overall, predictions with both models were not significantly different from measured emissions (P < 0.05) and were within 52 to 61% agreement with observations. Inclusion of data for organic matter characteristics improved model predictions of high (>30 mg m-2 h-1) N2O emissions, but tended to overestimate low emission rates (<20 mg N2O m-2 h-1). This work represents one of the first attempts to model feedyard N2O. Further refinement is needed to be useful for predicting spatial and temporal variations in feedyard N2O fluxes.

Future Plans

This work clearly identified that neither the magnitude nor the dynamics of N2O emissions from open-lot cattle systems were well understood. Five primary knowledge gaps/problem areas were identified, where current understanding is weak and further research is required. These include: (i) the need for accurate measurement of N2O emissions with appropriate and more standardized methods; (ii) improved understanding of the microbiology, chemistry, and physical structure of manure within feedyard pens that lead to N2O emissions; (iii) improved understanding of factors that influence feedyard N2O emissions, including manure H2O content, porosity, density, available nitrogen and carbon contents, environmental temperatures, and use of veterinary pharmaceuticals; (iv) development of cost-effective and practical mitigation strategies to decrease N2O emissions from pen surfaces, manure stockpiles, composting windrows, and retention ponds; and (v) improved process-based models that can accurately predict feedyard N2O emissions, evaluate mitigation strategies, and forecast future N2O emission trends.

Given the potential for future regulation of N2O emissions, feedyard managers, nutritionists, and researchers may play increasingly important roles in on-farm nitrogen management. Current management practices may need modification or refinement to balance production efficiency with environmental concerns. There is a need for data derived from both large-scale micrometerological measurement campaigns and small-scale chamber studies to assess the overall magnitude of feedyard N2O emissions and to determine key factors driving its production and emission. Refined empirical and process-based models based on manure physicochemical properties and weather could provide a dynamic approach to predict N2O losses from open-lot cattle systems.

Corresponding author (name, title, affiliation):

Heidi Waldrip, Research Chemist, USDA-ARS Conservation and Production Laboratory, Bushland, TX

Corresponding author email address

heidi.waldrip@ars.usda.gov

Other Authors

Rick Todd, Research Soil Scientist, USDA-ARS Conservation and Production Laboratory, Bushland, TX

David Parker, Agricultural Engineer, USDA-ARS Conservation and Production Research Laboratory, Bushland, TX

Al Rotz, Agricultural Engineer, USDA-ARS Pasture Systems and Watershed Management Research Unit, University Park, PA

Andy Cole, Animal Scientist, USDA-ARS Conservation and Production Research Laboratory (retired), Bushland, TX.

Ken Casey, Associate Professor, Texas A&M AgriLife Research, Amarillo, TX

Additional Information

“Nitrous Oxide Emissions from Open-Lot Cattle Feedyards: A Review”. Waldrip, H. M., Todd, R. W., Parker, D. B., Cole, N. A., Rotz, C. A., and Casey, K. D. 2016. J. Environ. Qual. 45:1797-1811. Open-access article available at:  https://dl.sciencesocieties.org/publications/jeq/pdfs/45/6/1797?search-r…

USDA-ARS Research on Feedyard Nitrogen Sustainability: http://www.beefresearch.org/CMDocs/BeefResearch/Sustainability_FactSheet…

Acknowledgements

This research was partially funded by the Beef Checkoff: http://www.beefboard.org/

The authors are solely responsible for the content of these proceedings. The technical information does not necessarily reflect the official position of the sponsoring agencies or institutions represented by planning committee members, and inclusion and distribution herein does not constitute an endorsement of views expressed by the same. Printed materials included herein are not refereed publications. Citations should appear as follows. EXAMPLE: Authors. 2017. Title of presentation. Waste to Worth: Spreading Science and Solutions. Cary, NC. April 18-21, 2017. URL of this page. Accessed on: today’s date.

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A Model Comparison of Daily N2O Flux with DayCent, DNDC, and EPIC


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Purpose 

Process-based models are increasingly used as tools for studying complex agroecosystem interactions and N2O emissions from agricultural fields. The widespread use of these models to conduct research and inform policy benefits from periodic model comparisons that assess the state of agroecosystem modeling and indicate areas for model improvement. The increasingly broad application of models requires an assessment of model performance using datasets that span multiple biogeophysical contexts. While limited in the capacity to identify specific areas for model improvement, general evaluations provide a critical perspective on the use of model estimates to inform policy and also identify necessary model improvements that require further evaluation.

What did we do? 

The objectives of this model comparison were to i) calibrate and validate three process-based models using a large dataset; ii) evaluate the performance of a multi-model ensemble to estimate observed data; and iii) construct a linear model to identify and quantify possible model bias in the estimation of soil N2O flux from agricultural fields. We selected three models that have been used to evaluate N2O emissions from agricultural fields: DayCent, DNDC, and EPIC. Using data from two field experiments over five years, we calibrated and validated each model using observations of soil temperature (n = 887), volumetric soil water content (VSWC) (n = 880), crop yield (n = 67), and soil N2O flux (n = 896). Our model validations and comparisons consisted of commonly conducted statistical evaluations of root mean squared error, correlation, and model efficiency. Additionally, the large sample sizes used here allowed for more robust linear regression models that offered additional insight into relationships between model estimations and observations of N2O flux. We hypothesized that such a linear model would indicate if there was model bias in estimations of soil N2O flux. Ensemble modeling can reduce the error associated with climate projections and has recently been applied to the estimation of N2O flux from agroecosystems. Thus, we also constructed a multi-model ensemble to evaluate the use of multiple models to improve estimates of soil N2O flux.

What have we learned? 

In a comparison of three process-based models, calibration to a large dataset produced favorable estimations of soil temperature, VSWC, average yield, and N2O flux when the models were evaluated using RMSE, R2, and the Nash-Sutcliffe E-statistic. However, an evaluation of linear regression models revealed a consistent bias towards underestimating high-magnitude daily N2O flux and cumulative N2O flux. Observations of soil temperature and VSWC were unable to significantly explain model bias. Calibration to available data did not result in consistent model estimation of additional system properties that contribute to N2O flux, which suggests a need for additional model comparisons that make use of a wide variety of data types. The major contribution of this work has been to identify a potential model bias and future steps required to evaluate its source and improve the simulation of nitrogen cycling in agroecosystems. Process-based models are powerful tools, and it is not our objective to undermine their past and future application. However, more work is left to be done in understanding the biogeophysical system that produces soil N2O and in harmonizing the process-based models that simulate that system and which are used to evaluate management and generate policy.

Future Plans 

Future work should test our findings in additional agroecological contexts to determine the extent to which a bias towards underestimating peak N2O flux persists. A meta-analysis of published data may be the most direct method for doing so. New datasets will need to be collected that represent simultaneous observations of multiple system properties (e.g. soil NO3-, soil NH4+, and heterotrophic respiration) from different soil layers and at increased temporal frequencies. Model developers should use these rich datasets to identify the source of N2O estimation bias and improve the structure and function of process-based models.

Corresponding author, title, and affiliation      

Richard K. Gaillard, Graduate Student, University of Wisconsin-Madison

Corresponding author email    

rgaillard@wisc.edu

Other authors  

Curtis D. Jones, Assistant Research Professor, University of Maryland; Pete Ingraham, Research Scientist, Applied Geosolutions;

Additional information               

sustainabledairy.org

Acknowledgements     

This material is based upon work that is supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, under award number 2013-68002-20525. Any opinions, findings, conclusions or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture.

Additional Authors:

Sarah Collier, Research Associate, University of Wisconsin-Madison;

Roberto Cesar Izaurralde, Research Professor, University of Maryland;

William Jokela, Research Scientist (retired), USDA-ARS;

William Osterholz, Research Associate, University of Wisconsin-Madison;

William Salas, President and Chief Scientist, Applied Geosolutions;

Peter Vadas, Research Scientist, USDA-ARS;

Matthew Ruark; Associate Professor; University of Wisconsin-Madison

The authors are solely responsible for the content of these proceedings. The technical information does not necessarily reflect the official position of the sponsoring agencies or institutions represented by planning committee members, and inclusion and distribution herein does not constitute an endorsement of views expressed by the same. Printed materials included herein are not refereed publications. Citations should appear as follows. EXAMPLE: Authors. 2017. Title of presentation. Waste to Worth: Spreading Science and Solutions. Cary, NC. April 18-21, 2017. URL of this page. Accessed on: today’s date.

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Greenhouse Gases and Agriculture (Self Study Lesson)

This is a self-guided learning lesson about greenhouse gases (GHG) and their connections to livestock and poultry production. It is useful for self-study and for professionals wishing to submit continuing education credits to a certifying organization. Anticipated time: 60 minutes. At the bottom of the page is a quiz that can be submitted and a score of 7 out of 10 or better will earn a certificate of completion. (Teachers/educators: visit the accompanying GHG curriculum materials page)

Module Topics

  1. Why does climate change?
  2. How does US agriculture to compare to other industries and worldwide agriculture?
  3. What greenhouse gases (GHG) are emitted by livestock and poultry farms?
  4. What are mitigation and adaptation strategies

What is Climate Change?

Download and read “Why Does Climate Change?” (PDF; 8 pages). Includes basics and terminology about natural and man-made drivers of climate change.

US Agriculture Comparisons to Other Industries and Worldwide Agriculture

Watch this short video “Agriculture and Greenhouse Gases: Some Perspective” (5 minutes). This also includes some very good reasons why farmers, ranchers, and ag professionals should care about the topic of climate change, regardless of political stances on solutions.

Greenhouse Gases Emitted by Livestock, Poultry. and Other Agricultural Activities

Watch this short video discussing the most important gases produced through livestock, poultry, and cropping activities on farms and ranches. (8 minutes)

Review the following fact sheet:

Mitigation and Adaptation

Watch this short video “Carbon, Climate Change, and Controversy” by Marshall Sheperd, University of Georgia (4 minutes)

Watch this video on “Mitigation and Adaptation: Connections to Agriculture” (13 minutes)

Quiz

When you have completed the above activities, take this quiz. If you score at least 7 of 10 correct, you will receive a certificate of completion via email. If you are a member of an organization that requires continuing education units (CEUs), we recommend that you submit your certificate to them for consideration as a self-study credit (each individual organization usually has a certification board that decides which lessons are acceptable). Go to quiz….

American Registry of Professional Animal Scientist (ARPAS) members can self-report their completion of this module at the ARPAS website.

Acknowledgements

Author: Jill Heemstra, University of Nebraska-Lincoln

Building Environmental Leaders in Animal Agriculture (BELAA) is a collaborative effort of the National Young Farmers Educational Association, University of Nebraska-Lincoln, and Montana State University. It was funded by the USDA National Institute for Food and Agriculture (NIFA) under award #2009-49400-05871. This project would not be possible without the Livestock and Poultry Environmental Learning Community and the National eXtension Initiative.

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Measuring Nitrous Oxide and Methane Emissions from Feedyard Pen Surfaces; Experience with the NFT-NSS Chamber Technique

Why Study Nitrous Oxide and Methane at Cattle Feedyards?

Accurate estimation of greenhouse gas emissions, including nitrous oxide and methane, from open beef cattle feedlots is an increasing concern given the current and potential future reporting requirements for GHG emissions. Research measuring emission fluxes of GHGs from open beef cattle feedlots, however, has been very limited. Soil and environmental scientists have long used various chamber based techniques, particularly non-flow-through – non-steady-state (NFT-NSS) chambers for measuring soil fluxes. Adaptation of this technique to feedyards presents a series of challenges, including spatial variability, presence of animals, chamber base installation issues, gas sample collection and storage, concentration analysis range, and flux calculations.

What did we do? 

Following an extensive review of the literature on measuring emissions from cropping and pasture systems, it was decide to adopt non-flow-through – non-steady-state (NFT-NSS) chambers as the preferred measurement methodology. However, the use of these NFT-NSS chambers had to be adapted for use in conditions of beef cattle feedyards and open corral dairies.

What have we learned? 

Trials of various techniques for sealing the chamber to the manure surface including piling soil/manure around the chamber and various weighted skirts were trial, however no technique was as good at sealing the chamber as a metal ring driven 50-75 mm into the underlying substrate.

Chamber bases could potentially injure animal in the pen and/or animal could disturb the measurement installation, so measurements were only conducted in recently vacated pens.

Gas samples were drawn from a septa in the chamber cap using a 20 ml polyethylene syringe and immediately injected into a 12 ml evacuated exetainer vial for transport, storage and analysis. Trials of alternative vials led to sample loss and contamination issues.

Gas samples were analyzed using a gas chromatograph equipped with ECD, FID and TCD detectors for nitrous oxide, methane and carbon dioxide determination, respectively.

The metal rings or bases must be installed at least 24 and preferably 48 hours before measurements are commenced as the disturbance caused when installing the bases will result in a temporarily enhanced emission flux.

Ten, 20 cm dia chambers constructed from PVC pipe caps are deployed in a pen and yield a reasonable approximation of the average emission fluxes from the pen.

The range of gas concentrations measured in the chamber at the end of a 30 minute deployment was up to 2 orders of magnitude greater than that typically measured in cropping systems research. This required careful choice of calibration gas concentrations and calibration of the gas chromatograph. The response of the ECD detector used for determining N2O concentration may not be linear over the entire range experienced.

The rate of increase in concentration in the chamber is often curvilinear in form and a quadratic approach was adopted for determination of the flux rate.

Future Plans 

On-going studies are quantifying N2O and CH4 flux rates from pen surfaces in a cattle feedlots under varying seasonal conditions; further work is identifying contributing factors.

Authors

Kenneth D. Casey, Associate Professor at Texas A&M AgriLife Research, Amarillo TX kdcasey@ag.tamu.edu

Heidi M. Waldrip, Research Soil Scientist at USDA ARS CPRL, Bushland TX; Richard W. Todd, Research Soil Scientist at USDA ARS CPRL, Bushland TX; and N. Andy Cole, Research Soil Scientist at USDA ARS CPRL, Bushland TX;

Additional information 

For further information, contact Ken Casey, 806-677-5600

Acknowledgements

Research was partially funded from USDA NIFA Special Research Grants

The authors are solely responsible for the content of these proceedings. The technical information does not necessarily reflect the official position of the sponsoring agencies or institutions represented by planning committee members, and inclusion and distribution herein does not constitute an endorsement of views expressed by the same. Printed materials included herein are not refereed publications. Citations should appear as follows. EXAMPLE: Authors. 2015. Title of presentation. Waste to Worth: Spreading Science and Solutions. Seattle, WA. March 31-April 3, 2015. URL of this page. Accessed on: today’s date.

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Factors Affecting Nitrous Oxide Emissions Following Subsurface Manure Application

[Abstract] Subsurface manure application is theoretically susceptible to greater denitrification losses and nitrous oxide (N2O) emissions compared to surface application methods – primarily attributed to manure being placed in a more anaerobic environment. A review of field studies suggest N2O emissions typically range from 0.1% to 3% of total applied N from subsurface application methods, but there is considerable variation in emissions depending on pre- and post-application soil moisture conditions, readily-available carbon content in manure compared to background levels in soil, localized nitrogen form and oxygen concentration at the application site, and application depth. This paper will summarize peer-reviewed literature of field studies that quantify N2O emissions subsequent to subsurface manure application and identify the most prominent determining factors cited by authors.

Why Study Nitrous Oxide Emissions of Manure?

Ammonia abatement efficiencies of up to 90 percent have been documented with subsurface application and incorporation of animal manures compared to conventional surface application methods. While reducing ammonia emissions has positive implications for air and water quality, a portion of the nitrogen conserved may come at the expense of increased nitrous oxide emissions produced during denitrification and nitrification processes in the soil. As a greenhouse gas 300 times more potent than carbon dioxide at trapping heat, nitrous oxide has been linked to anthropogenic climate change and depletion of stratospheric ozone. Release of nitrous oxide from agriculturally-productive soils into the atmosphere also represents a loss of crop nutrients. Understanding the circumstances and manageable factors that contribute to nitrous oxide formation in soils subsequent to manure application is important for retaining crop nutrients and preventing greenhouse gas emissions.

What did we do?

A literature review was performed to investigate the factors that contribute to nitrous oxide emissions following subsurface application of animal manure to both grassland and arable land, compare results from different application techniques, and examine the conditions and circumstances that lead to nitrous oxide emissions.

What have we learned?

Several studies demonstrate significant increases in nitrous oxide emissions (from 0.1 to 3 percent) attributable to factors including increasing soil moisture content, high concentrations of readily-available carbon in manure substrate, increased nitrate concentration in soil, shallow application depth, high soil temperature, and ambient conditions during and immediately following application (table 1). Other studies show no difference in nitrous oxide emissions as compared to surface application methods. Reasons that subsurface application techniques will not necessarily result in greater nitrous oxide emissions were: 1) the length of the diffusion path from the site of denitrification to the soil surface may lead to a greater portion of denitrified nitrogen being emitted as nitrogen gas; 2) the soil moisture conditions and aeration level at the time of application may not be suitable for increased nitrous oxide production; 3) prior to manur e application, soils may already contain readily-metabolizable carbon and mineral nitrogen, thus any increase in nitrous oxide emission following application may not have a significant impact; and 4) weather events subsequent to manure application may effect soil moisture content and water-filled-pore-space, thereby affecting nitrous oxide emissions. Several studies document nitrous oxide emissions due to subsurface application methods (including manure incorporation and shallow injection) but research comparing nitrous oxide emissions from different subsurface application techniques and application depth is limited. Lack or absence of data in literature about manure chemistry, nitrogen application rates, application technique or method, as well as soil and atmospheric conditions during and after application made it more difficult to draw specific conclusions on factors affecting nitrous oxide emissions from subsurface-applied manure.

Further research is needed to determine the environmental and economic tradeoffs of implementing subsurface manure application methods for abatement of NH3 considering different future greenhouse gas emissions and market scenarios. Recent work suggests a link between denitrifier community density, organic C, and N2O emissions. Characterization of these biological mechanisms and identification of genetic markers for key enzymes should continue, particularly with respect to various subsurface manure application techniques, different manure types and N application rates, soil types, environmental conditions, and soil chemistry. Subsurface application depth plays an important role in determining the proportion of N2O to N2 emitted during denitrification; however, the number of field studies that examine the impact of application depth is limited. More research is needed to determine optimal manure application depth as influenced by soil type, soil chemistry, timing of application, and vegetative cover. Finally, future research on subsurface manure application will allow existing and future prediction models to improve estimation of annual N2O emissions at landscape scale and airshed levels. Refinement of greenhouse gas inventories, including N2O emissions from agricultural production systems, will assist agriculture producers, scientists, and policy makers in making informed decisions on greenhouse gas emission mitigation.

research articles reporting factors of Nitrous Oxide

Future Plans

Future agricultural greenhouse gas regulations and/or carbon market incentives have potential implications for agricultural producers, including the method and timing of manure application. Controlled, replicated, and well-documented research on subsurface manure application and subsequent nitrous oxide release is critical for estimating the costs and benefits of different manure application techniques.

Authors

David W. Smith, Extension Program Specialist, Texas A&M AgriLife Extension DWSmith@ag.tamu.edu

Dr. Saqib Mukhtar, Professor and Associate Department Head for Extension, Texas A&M AgriLife Extension

Additional information

The publication ‘Estimation and Attribution of Nitrous Oxide Emissions Following Subsurface Application of Animal Manure: A Review’ has been accepted for publication in Transactions of the ASABE.

Acknowledgements

Funding for this effort provided by USDA-NIFA grant No. 2011-67003-30206.

The authors are solely responsible for the content of these proceedings. The technical information does not necessarily reflect the official position of the sponsoring agencies or institutions represented by planning committee members, and inclusion and distribution herein does not constitute an endorsement of views expressed by the same. Printed materials included herein are not refereed publications. Citations should appear as follows. EXAMPLE: Authors. 2015. Title of presentation. Waste to Worth: Spreading Science and Solutions. Seattle, WA. March 31-April 3, 2015. URL of this page. Accessed on: today’s date.

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Measuring nitrous oxide emissions from a Wisconsin dairy forage cropping system

Nitrous oxide emitted from cropland constitutes a significant component of the agricultural sector’s overall greenhouse gas footprint. In order to accurately evaluate mitigation strategies, predict impacts, and model system behavior under future climate scenarios, it is essential to have access to flux measurements collected under regionally relevant conditions of soil, weather, and management strategies. As part of the Climate Change Mitigation and Adaptation in Dairy Production Systems of the Great Lakes Region USDA Coordinated Agricultural Project, we are measuring nitrous oxide flux from a typical dairy forage rotation in south-central Wisconsin. The rotation consists of one year of corn and three years of alfalfa, receiving liquid dairy manure fertilization in corn and alfalfa establishment years. Fluxes have been tracked over two growing seasons, and comparisons are possible between years as well as between phases of the rotation. Ultimately this data will be used to calibrate models for use in footprinting and benchmarking efforts and in predicting future productivity and resilience of dairy-based systems.

Author

Collier   Sarah     smcollier@wisc.edu        University of Wisconsin-Madison

The authors are solely responsible for the content of these proceedings. The technical information does not necessarily reflect the official position of the sponsoring agencies or institutions represented by planning committee members, and inclusion and distribution herein does not constitute an endorsement of views expressed by the same. Printed materials included herein are not refereed publications. Citations should appear as follows. EXAMPLE: Authors. 2015. Title of presentation. Waste to Worth: Spreading Science and Solutions. Seattle, WA. March 31-April 3, 2015. URL of this page. Accessed on: today’s date.

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