In the Manure and Soil Biology roundtable, our goal was to discover the influence of manure, both positive and negative, on soil biology. We discussed if certain fields will produce more soil biology benefits than others and whether timing of application affects these benefits. Finally, we debated whether we can derive an economic value for manure beyond its nutrient value. Field experiences and observations related to the value of manure as well as what farmers still need related to soil building with manure were reviewed. This dialogue was the second in a four part series discussing the current state of our knowledge relative to manure’s impact on soil health.